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品种反应性 主要应用 宿主 格式 抗体类型 H WB M Ascites Monoclonal Antibody 描述 产品目录编号 MAB10765 描述 Anti-Red Fluorescent Protein Antibody, clone 1DS-1A6 | MAB10765 Alternate Names - Red fluorescent protein
背景信息 Since the development of Green fluorescent protein’s (GFP) use as a protein tag in chimeric proteins, fluorescent proteins have become widely used and useful biotechnological tools. The isolation of Red Fluorescent proteins have opened up a broader color spectrum for use in research. These proteins can be fused with a wide variety of proteins at either the N- or C-terminus, and have been expressed as fluorescent markers in most cell types. These markers can be used as noninvasive markers in both living organisms and cells. 产品信息 格式 Ascites 控制 - Cos cell lysate with overexpression of RFP
演示 Unpurified mouse monoclonal ascites with 0.05% sodium azide. 应用 主要应用 - Western Blotting
生物信息 免疫原品种 Recombinant protein corresponding to sea anenome Red fluorescent protein. 表位 Unknown 克隆 1DS-1A6 宿主 Mouse 特异性 This antibody recognizes Red fluorescent protein. 同种型 IgG1κ 品种反应性 Human Species Reactivity Note Demonstrated to react with sea anemone. 抗体类型 Monoclonal Antibody 基因符号 - RFP
纯化方法 Unpurified UniProt编号 - Q9U6Y8
UniProt汇总 FUNCTION: Thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs. In deeper water, the fluorescence may be to convert blue light into longer wavelengths more suitable for use in photosynthesis by the microalgal symbionts.
SUBUNIT STRUCTURE: Homotetramer.
PTM: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Xaa-N and Gly-N+2, oxidation of Tyr-N+1 to didehydrotyrosine, and formation of a double bond to the alpha-amino nitrogen of residue Xaa-N. Maturation of the chromophore requires nothing other than molecular oxygen.
BIOTECHNOLOGICAL USE: Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
SEQUENCE SIMILARITIES: Belongs to the GFP family.
BIOPHYSICOCHEMICAL PROPERTIES: Absorption:
Exhibits a smaller absorbance peak at 494 nm. The broad fluorescence emission spectrum peaks at 583 nm.
Abs(max)=558 nm产品使用声明 质量保证 Evaluated by Western Blot in Cos cell lysate with overexpression of RFP.
Western Blot Analysis: 1:1,000 dilution of this antibody detected Red fluorescent protein on 10 µg of Cos cell lysate with overexpression of RFP.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
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