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merck millipore,默克密理博,MAB1061,Anti-Bone Sialoprotein II Antibody, CT, clone ID1.2
产品名称:Anti-Bone Sialoprotein II Antibody, CT, clone ID1.2
产品型号:MAB1061
This Anti-Bone Sialoprotein II Antibody, C-terminus, clone ID1.2 is validated for use in ELISA, RIA, WB, IC, IH(P) for the detection of Bone Sialoprotein II.
merck millipore,默克密理博,MAB1061,Anti-Bone Sialoprotein II Antibody, CT, clone ID1.2
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H ICC, ELISA, WB M Purified Monoclonal Antibody 描述 产品目录编号 MAB1061 Replaces AB1855 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Bone Sialoprotein II Antibody, CT, clone ID1.2 Alternate Names - Integrin-binding Sialoprotein
- BSPII
背景信息 Bone Sialoprotein II is one of the major noncollagenous proteins in the extracellular matrix of bone. It is a phosphorylated glycoprotein with an approximate molecular weight of 70 kDa. The protein has been found in osteoblasts and osteocytes. Diseases concerning bone turnover, pathological bone alterations, as well as the rapid increase of osteoporosis make it necessary to establish new markers. Bone Sialoprotein is discussed as a potential serum marker for monitoring bone remodeling. 产品信息 格式 Purified 演示 Purified immunoglobulin from culture supernatant. Lyophilized from 50 mM TRIS, pH7.4. 应用 应用 This Anti-Bone Sialoprotein II Antibody, C-terminus, clone ID1.2 is validated for use in ELISA, RIA, WB, IC, IH(P) for the detection of Bone Sialoprotein II. 主要应用 - Immunocytochemistry
- ELISA
- Western Blotting
应用说明 Western blot: (1μg/mL)
Immunohistochemistry on paraffin embedded tissue sections: (1μg/mL)
Immunocytochemistry: (1:100)
ELISA: (1μg/mL)
RIA
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Bone Sialoprotein isolated from human bones. 表位 C-terminus 克隆 ID1.2 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Recombinant and native human bone sialoprotein II (BSPII). Does not react with osteopontin (BSPI) or osteonectin. 同种型 IgG1 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_004967.2
Entrez基因汇总 The protein encoded by this gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. 基因符号 - BSP
- IBSP
- BNSP
- SP-II
- BSP-II
非反应性品种 - Rat
- Bovine
- Pig
UniProt编号 - P21815
UniProt汇总 FUNCTION: SwissProt: P21815 # Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment.
SIZE: 317 amino acids; 35018 Da
SUBCELLULAR LOCATION: Secreted.
PTM: N-glycosylated; glycans consist of sialated and core- fucosylated bi-, tri- and tetraantennary chains. & O-glycosylated at eight sites; mucin-type glycans contain Gal, GlcNAc, GalNAc and terminal NeuAc.
MISCELLANEOUS: It is possible that the segments of clustered carboxyl groups mediate the strong binding to hydroxyapatite.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain lyophilized material at 2-8°C. Reconstitute with 100μL sterile distilled water and store at -20°C in undiluted aliquots up to 12 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µg
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