merck millipore,默克密理博,LIF2010,Leukemia Inhibitory Factor Protein, Recombinant mouse

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merck millipore,默克密理博,LIF2010,Leukemia Inhibitory Factor Protein, Recombinant mouse
产品名称：Leukemia Inhibitory Factor Protein, Recombinant mouse
产品型号：LIF2010
Recombinant mouse Leukemia Inhibitory Factor (LIF) protein is biologically active and suitable for cell culture applications.

merck millipore,默克密理博,LIF2010,Leukemia Inhibitory Factor Protein, Recombinant mouse

产品介绍

merck millipore,默克密理博,LIF2010,Leukemia Inhibitory Factor Protein, Recombinant mouse

重要规格表

主要应用	品种	来源	纯度
CULT	Mouse	mLIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moeity with thrombin and purified by HPLC chromatography.	Greater than 95% by analytical HPLC and SDS-PAGE. Endotoxin level is less than 0.1 ng per mg of LIF. Tested negative in both aseptic and microplasmic tests.

描述
产品目录编号	LIF2010
品牌系列	Chemicon®
商名	Chemicon
描述	Leukemia Inhibitory Factor Protein, Recombinant mouse
概述	Leukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo. Mouse LIF is a 22 kDa protein containing 203 amino acid residues.
Alternate Names	D Factor LIF Differentiation-stimulating Factor

产品信息
演示	Liquid in PBS, pH 7.4 and 0.02% Tween 20. No preservativies added.

应用
应用	Recombinant mouse Leukemia Inhibitory Factor (LIF) protein is biologically active and suitable for cell culture applications.
主要应用	Cell Culture
应用说明	RECOMMENDED QC PROTOCOL M1 Bioassay 1. The M1 bioassay is performed using in vitro semi-solid agar cultures, which contain approximately 100 cells in 1 mL volumes of DME containing 20 % FCS in 0.3% agar. 2. Add 100 μL of sample or mLIF (10(E4) units/mL in 5% FCS in isotonic saline) in two-fold serial dilutions in duplicate to 35 mm petri dishes. 3. Add 100 μL of 5% FCS in isotonic saline to two control slides. 4. Incubate at 37°C in fully humidified atmosphere of 10% CO2 in air for 7 days. 5. Score the number of colonies that show differentiation (note: 50 units is defined as the amount of activity which results in 50% of the colonies being differentiated). Manufactured by CHEMICON International, Inc. LIF is protected under US Patent nos. 5,187,077, 5,427,925, 5,443,825, 5,750,654 and 6,261,548, European Patent no. 0285 448 and related foreign patents and is not available for resale.

生物信息
浓缩	10 μg/mL
纯度	Greater than 95% by analytical HPLC and SDS-PAGE. Endotoxin level is less than 0.1 ng per mg of LIF. Tested negative in both aseptic and microplasmic tests.
来源	mLIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moeity with thrombin and purified by HPLC chromatography.
品种	Mouse
特异活性	The activity of mouse LIF is determined by the ability to induce differentiation of murine M1 myeloid leukemic cells. The minimum detectable concentration of mouse LIF in this assay is 0.5 ng/mL. The specific activity is >=1 x 10(E8) units/mg, where 50 units is defined as the amount of mouse LIF required to induce differentiation in 50% of the M1 colonies in 1 mL agar cultures.
Entrez基因编号	NM_008501
Entrez基因汇总	Leukaemia inhibitory factor is a cytokine that induces macrophage differentiation. Neurotransmitters and neuropeptides, well known for their role in the communication between neurons, are also capable of activating monocytes and macrophages and inducing chemotaxis in immune cells. LIF signals through different receptors and transcription factors. LIF in conjunction with BMP2 acts in synergy on primary fetal neural progenitor cells to induce astrocytes.
UniProt编号	P09056
UniProt汇总	FUNCTION: SwissProt: P09056.1 # LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. SIZE: 203 amino acids; 22,287 Da SUBCELLULAR LOCATION: Secreted. SIMILARITY: SwissProt: P09056.1 ## Belongs to the LIF/OSM family.

产品使用声明
使用声明	LABEL LICENSE - USE RESTRICTED TO RESEARCH USE ONLY Leukemia Inhibitory Factor ("LIF") is protected by US Patents No. 5,443,825 and 5,750,654 and Canada Patent No. 1,341,581 and 1,341,469 owned by AMRAD Corporation Ltd. and licensed exclusively to Millipore for research uses. The use of this product is limited for research uses only and not for any commercial purposes, such as making or developing other research products from this product or use of this product as a component in a research kit. This product is not to be used in humans or for any diagnostic purposes. The purchaser may refuse to accept the conditions of this label license by returning this product unopened and will receive a full refund of the purchase price paid. For-profit entities or commercial research entities purchasing this product shall be required to execute a separate commercial license with Millipore within three months of purchase. Researchers may not transfer this product or its derivatives to researchers performing commercial research at other Nonprofit Organizations, or to For-Profit Organizations, without the express written consent of Millipore, and without those entities having an express license from Millipore for the use of the product. Other than as specifically provided herein, a transferee of this product shall have no right to transfer this product, or its derivatives to any other person or entity. Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

使用声明

LABEL LICENSE - USE RESTRICTED TO RESEARCH USE ONLY

Leukemia Inhibitory Factor ("LIF") is protected by US Patents No. 5,443,825 and 5,750,654 and Canada Patent No. 1,341,581 and 1,341,469 owned by AMRAD Corporation Ltd. and licensed exclusively to Millipore for research uses. The use of this product is limited for research uses only and not for any commercial purposes, such as making or developing other research products from this product or use of this product as a component in a research kit. This product is not to be used in humans or for any diagnostic purposes.

The purchaser may refuse to accept the conditions of this label license by returning this product unopened and will receive a full refund of the purchase price paid.

For-profit entities or commercial research entities purchasing this product shall be required to execute a separate commercial license with Millipore within three months of purchase. Researchers may not transfer this product or its derivatives to researchers performing commercial research at other Nonprofit Organizations, or to For-Profit Organizations, without the express written consent of Millipore, and without those entities having an express license from Millipore for the use of the product. Other than as specifically provided herein, a transferee of this product shall have no right to transfer this product, or its derivatives to any other person or entity.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain at 2-8°C for 6 - 12 months. Further dilutions should be made into buffer or medium to which protein (e.g., 1% BSA) or Tween 20 has been added. Manufactured by CHEMICON International, Inc. LIF is protected under US Patent nos. 5,187,077, 5,427,925, 5,443,825, 5,750,654 and 6,261,548, European Patent no. 0285 448 and related foreign patents and is not available for resale.