merck millipore,默克密理博,05-583,Anti-CFTR Antibody, clone M3A7

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merck millipore,默克密理博,05-583,Anti-CFTR Antibody, clone M3A7
产品名称：Anti-CFTR Antibody, clone M3A7
产品型号：05-583
Detect CFTR using this Anti-CFTR Antibody, clone M3A7 validated for use in IH, IP & WB.

merck millipore,默克密理博,05-583,Anti-CFTR Antibody, clone M3A7

产品介绍

merck millipore,默克密理博,05-583,Anti-CFTR Antibody, clone M3A7

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	WB, IHC	M	Purified	Monoclonal Antibody

描述
产品目录编号	05-583
品牌系列	Upstate
商名	Upstate
描述	Anti-CFTR Antibody, clone M3A7
Alternate Names	cystic fibrosis transmembrane conductance regulator, ATP-binding cassette (sub-family C, member 7) ATP-binding cassette sub-family C, member 7 ATP-binding cassette transporter sub-family C member 7 cAMP-dependent chloride channel cystic fibrosis transmembrane conductance regulator cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7) cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C member 7)
背景信息	CFTR (Cystic Fibrosis Transmembrane Regulator) is an integral membrane protein shown to be involved in the transport of chloride ions. It is found on the surface of the epithelial cells lining the lungs and other organs. CFTR defects are the cause of cystic fibrosis. Cystic Fibrosis (CF) is a common lethal genetic disease caused by mutations of the gene coding for the cystic fibrosis transmembrane conductance factor, a cAMP regulated chloride channel. Approximately 70% of all CF cases share the deletion of a phenylalanine at position 508 (delta F508), which results in abnormal chloride transport. Since the CF mutation is lethal, most often by lung and liver disease, it raises the question of why this genetic disease remains as common as it is.

产品信息
格式	Purified
控制	T84 cell lysate.
演示	Purified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol.

应用
应用	Detect CFTR using this Anti-CFTR Antibody, clone M3A7 validated for use in IH, IP & WB.
主要应用	Western Blotting Immunohistochemistry
应用说明	Immunoprecipitation: This antibody has been reported to immunoprecipitate CFTR. (Kartner, N., 1998.) Immunohistochemistry: This antibody has been reported to immunostain CFTR in human pancreatic tissue sections. (Kartner, N., 1998.)

生物信息
免疫原品种	GST-fusion protein corresponding to residues 1197-1480 of human cystic fibrosis transmembrane conductance regulator (CFTR). The epitope maps within amino acids 1365-1395. Clone M3A7.
表位	a.a. 1365-1395
克隆	M3A7
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	This antibody recognizes CFTR, Mr 170 kDa.
同种型	IgG1
品种反应性	Human
Species Reactivity Note	Human. Other species not tested.
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_000492
Entrez基因汇总	This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily that is involved in multi-drug resistance. The encoded protein functions as a chloride channel and controls the regulation of other transport pathways. Mutations in this gene are associated with the autosomal recessive disorders cystic fibrosis and congenital bilateral aplasia of the vas deferens. Alternatively spliced transcript variants have been described, many of which result from mutations in this gene.
基因符号	TNR-CFTR CFTR CFTR/MRP ABCC7 CBAVD dJ760C5.1 CF MRP7 ABC35
纯化方法	Protein G purfied
UniProt编号	P13569
UniProt汇总	FUNCTION: SwissProt: P13569 # Involved in the transport of chloride ions. May regulate bicarbonate secretion and salvage in epithelial cells by regulating the SLC4A7 transporter. SIZE: 1480 amino acids; 168142 Da SUBUNIT: Interacts with SHANK2 (By similarity). Interacts with SLC9A3R1, MYO6 and GOPC. Interacts with SLC4A7 through SLC9A3R1. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Found on the surface of the epithelial cells that line the lungs and other organs. DOMAIN: SwissProt: P13569 The PDZ-binding motif mediates interactions with GOPC and with the SLC4A7, SLC9A3R1/EBP50 complex. DISEASE: SwissProt: P13569 # Defects in CFTR are the cause of cystic fibrosis (CF) [MIM:219700]; also known as mucoviscidosis. CF is the most common genetic disease in the Caucasian population, with a prevalence of about 1 in 2000 live births. Inheritance is autosomal recessive. CF is a common generalized disorder of exocrine gland function which impairs clearance of secretions in a variety of organs. It is characterized by the triad of chronic bronchopulmonary disease (with recurrent respiratory infections), pancreatic insufficiency (which leads to malabsorption and growth retardation) and elevated sweat electrolytes. & Defects in CFTR are the cause of congenital bilateral absence of the vas deferens (CBAVD) [MIM:277180]. CBAVD is an important cause of sterility in men and could represent an incomplete form of cystic fibrosis, as the majority of men suffering from cystic fibrosis lack the vas deferens. SIMILARITY: Belongs to the ABC transporter family. CFTR transporter (TC 3.A.1.202) subfamily. & Contains 2 ABC transmembrane type-1 domains. & Contains 2 ABC transporter domains.

产品使用声明
质量保证	Routinely evaluated by western blot on human T84 colon carcinoma epithelial RIPA cell lysates or from CFTR-transfected BHK2. Western Blot Analysis: 0.5-2 µg/mL of this lot detected CFTR from 20-50 µg of human T84 colon carcinoma epithelial RIPA cell lysates. 0.5-2 µg/mL of a previous lot detected CFTR from CFTR-transfected BHK (Haardt, M., 1999). Note: Do not boil the lysate. Instead incubate at 37°C for 30 minutes. CFTR can run as a diffuse protein on SDS-PAGE.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

Routinely evaluated by western blot on human T84 colon carcinoma epithelial RIPA cell lysates or from CFTR-transfected BHK2.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected CFTR from 20-50 µg of human T84 colon carcinoma epithelial RIPA cell lysates. 0.5-2 µg/mL of a previous lot detected CFTR from CFTR-transfected BHK (Haardt, M., 1999).
Note: Do not boil the lysate. Instead incubate at 37°C for 30 minutes. CFTR can run as a diffuse protein on SDS-PAGE.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.