merck millipore,默克密理博,IF005,Interferon-γ Protein, Recombinant mouse

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merck millipore,默克密理博,IF005,Interferon-γ Protein, Recombinant mouse
产品名称：Interferon-γ Protein, Recombinant mouse
产品型号：IF005
The Interferon-gamma protein (or IFN-gamma protein) is a regulatory protein produced by activated NK cells & CD4+TCRalpha/beta+, CD8+TCRalpha/beta+ & TCRgamma/delta+ T cells.

merck millipore,默克密理博,IF005,Interferon-γ Protein, Recombinant mouse

产品介绍

merck millipore,默克密理博,IF005,Interferon-γ Protein, Recombinant mouse

重要规格表

主要应用	品种	来源	纯度
CULT	Mouse	Mouse, expressed in E.coli	Greater than 98% by SDS-PAGE and HPLC analysis. Endotoxin level is less than 0.1 ng per μg (1EU/μg) of IFN-gamma.

描述
产品目录编号	IF005
品牌系列	Chemicon®
商名	Chemicon
描述	Interferon-γ Protein, Recombinant mouse
概述	Interferon-gamma (IFN-gamma) is a regulatory protein produced by activated NK cells and CD4+TCRalpha/beta+, CD8+TCRalpha/beta+, and TCRgamma/delta+ T cells. The biological activities of IFN-gamma include the specific binding to a single class of high affinity receptors. In addition, IFN-gamma can upgrade the number of lymphoid cell functions including the antimicrobial and antitumor responses of macrophages, NK cells, and neutrophils. Recombinant mouse IFN-gamma is a 15.6 kDa protein containing 134 amino acid residues.

产品信息
演示	Sterile filtered, lyophilized from 10mM Sodium Phosphate, pH 7.0 + 25mM NaCl.

应用
应用	The Interferon-gamma protein (or IFN-gamma protein) is a regulatory protein produced by activated NK cells & CD4+TCRalpha/beta+, CD8+TCRalpha/beta+ & TCRgamma/delta+ T cells.
主要应用	Cell Culture
应用说明	For most in vitro applications, IFN-gamma exerts its biological activity in the concentration range of 0.1 to 10.0 ng/mL.

生物信息
纯度	Greater than 98% by SDS-PAGE and HPLC analysis. Endotoxin level is less than 0.1 ng per μg (1EU/μg) of IFN-gamma.
来源	Mouse, expressed in E.coli
品种	Mouse
特异活性	The ED50 as determined by a cytopathic effect inhibition assay with mouse NIH 3T3 cells challenged VSV is <= 0.1 ng/mL, corresponding to a specific activity of >= 1.0 x 10E7 units/mg.
UniProt编号	P01580 P01579
UniProt汇总	FUNCTION: SwissProt: P01579 # Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. SIZE: 166 amino acids; 19348 Da SUBUNIT: Homodimer. SUBCELLULAR LOCATION: Secreted. TISSUE SPECIFICITY: Released primarily from activated T lymphocytes. PTM: Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. DISEASE: SwissProt: P01579 # In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. SIMILARITY: SwissProt: P01579 ## Belongs to the type II (or gamma) interferon family.
细胞系类型	Epithelial Cells

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	The lyophilized protein is stable for a few weeks at room temperature, but is best stored at -20°C. After a quick spin, reconstitute with Sodium Phosphate, pH 8.0 to a concentration of 1 mg/mL. Further dilutions should be made into RPMI + 10% FCS or 5% HAS and stored at 2-8ºC for one week or -20ºC for future use. Reconstituted IFN-gamma should be stored in undiluted aliquots at -20°C for up to six months from date of receipt.

储存和货运信息

存储条件

The lyophilized protein is stable for a few weeks at room temperature, but is best stored at -20°C. After a quick spin, reconstitute with Sodium Phosphate, pH 8.0 to a concentration of 1 mg/mL. Further dilutions should be made into RPMI + 10% FCS or 5% HAS and stored at 2-8ºC for one week or -20ºC for future use. Reconstituted IFN-gamma should be stored in undiluted aliquots at -20°C for up to six months from date of receipt.