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merck millipore,默克密理博,AQ301R,Sheep Anti-Rabbit IgG Antibody, F(ab')2, Rhodamine conjugate
产品名称：Sheep Anti-Rabbit IgG Antibody, F(ab')2, Rhodamine conjugate
产品型号：AQ301R
Sheep anti-Rabbit IgG Antibody, F(ab')2, Rhodamine conjugate is an antibody against Rabbit IgG for use in IF.
merck millipore,默克密理博,AQ301R,Sheep Anti-Rabbit IgG Antibody, F(ab')2, Rhodamine conjugate
- 产品介绍
描述 产品目录编号 AQ301R 品牌系列 Chemicon®
商名 - Chemicon
描述 Sheep Anti-Rabbit IgG Antibody, F(ab')2, Rhodamine conjugate 概述 The reagent is an anti-rabbit antibody F(ab')2 fragment from sheep. The F(ab')2 fragments are obtained by pepsin cleavage. The fragments are coupled to 5(6)-carboxy-rhodamine 101-N-hydroxysuccinimide ester (rhodamine) and stabilized in buffer.The molar ratio of rhodamine: protein is between 2-3.
PURIFICATION: The antibody is isolated from antisera by immunoaffinity chromatography using antigen coupled to agarose beads.产品信息 格式 Rhodamine (TRITC) 演示 Lyophilized from 0.02M Sodium phosphate, 0.25M NaCl, pH 7.1 with 15 mg/mL BSA and 0.1% sodium azide.
RECONSTITUTION:
Reconstitute with 1 mL of sterile distilled water.应用 应用 Sheep anti-Rabbit IgG Antibody, F(ab')2, Rhodamine conjugate is an antibody against Rabbit IgG for use in IF. 主要应用 - Immunofluorescence
应用说明 The reagent allows a reliable and sensitive detection of rabbit IgG immunohistochemistry, respectively immunocytochemistry, especially when interference of immunoglobulins of other species than rabbit may occur. In addition, the absence of the "sticky" Fc portion avoids unspecific binding. Thus, the reagent is particularly suitable for use on sections and cells.
RECOMMENDED CONCENTRATION:
20-50 μg/mL. It is recommended that the antibody be diluted with PBS, pH 7.1 containing 15 mg/mL BSA.
Optimal working concentrations must be determined by the end user.生物信息 宿主 Sheep 特异性 The reaction profile of the antibody was examined in an ELISA system: micro titer plates were coated with antigen and potentially cross-reacting IgG of other species in identical concentrations, incubated with anti-rabbit IgG-biotin and detected with a streptavidin-enzyme conjugate. In this system the antibody shows the following reaction profile: No cross-reactivity (<0.01%) with Ig from human, porcine, bovine, goat, mouse and fetal calf serum (FCS). Weak reactivity (<1%) with Ig from horse and cross-reactivity (<5%) with Ig from rat and guinea pig.
WAVE LENGTH:
Absorption peak = 550 nm, Emission peak, 570 nm.品种反应性 Rabbit 抗体类型 Polyclonal Antibody 产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 When stored protected from light, the lyophilized antibody-conjugate is stable at 2-8°C.When stored protected from light the reconstituted antibody conjugate solution is stable for 2 months at 2-8°CThe solution should be stored in aliquots at -20°C.Repeated freezing and thawing should be avoided. 包装信息 数量 200 µg
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下一件merck millipore产品：merck millipore,默克密理博,05-636-I,Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301
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