merck millipore,默克密理博,05-636-I,Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301

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Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	ChIP, WB, ICC	M	Purified	Monoclonal Antibody

描述
产品目录编号	05-636-I
描述	Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301
Alternate Names	H2AXS139p Histone H2A.x H2a/x H2A.XS139p
背景信息	The histone H2A.X protein is a variant member of the H2A family of histones that is distinguished from other H2A histones by a unique carboxy-terminal sequence. This unique sequence is highly conserved throughout eukaryotic evolution and is rapidly phosphorylated by ATM or ATR at the fourth residue from the carboxy-terminus (Serine 139 in mammalian H2A.X) in response to DNA double-strand breaks (DSBs). Phosphorylation of H2A.X is important in the formation of a stable repair complex at the site of DNA damage. H2A.X phosphorylation is a very rapid response to DNA damage, occurring within as little as one minute after exposure to ionizing radiation. Phosphorylation of H2A.X occurs irrespective of the cause of the DNA DSBs and phospho-H2A.X has been observed in response to environmental stresses that result in DSBs as well as programmed cellular events, including DNA rearrangement and apoptosis.

产品信息
格式	Purified
演示	Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

应用
应用	Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301 is a highly specific mouse monoclonal antibody, that targets Histone H2A.X & has been tested in western blotting, ICC, ChIP & Immunofluorescence.
主要应用	Chromatin Immunoprecipitation (ChIP) Western Blotting Immunocytochemistry
应用说明	Immunocytochemistry Analysis: A 1:50-250 dilution from a representative lot detected phospho-Histone H2A. X (Ser139) in HeLa and A431 cells. Previous lot has been demonstrated to work in Immunofluorescence and Chromatin Immunoprecipitation: See reference (Meier, Andreas et al., 2007) Western Blotting Analysis: 0.05-1 μg/mL of this lot detected phosphorylated histone H2A.X (Ser139) in acid extracted histone lysates from Jurkat cells treated with 0.5 μM staurosporine (Catalog # 19-123). Immunocytochemistry: 2 μg/mL of a previous lot of antibody detected phosphorylated histone H2A.X in HeLa cells treated with 0.5μM staurosporine for 4-6 hours.

生物信息
免疫原品种	KLH-conjugated linear peptide corresponding to human phospho-Histone H2A. X (Ser139).
克隆	JBW301
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
同种型	IgG1κ
品种反应性	RatHumanMouse
抗体类型	Monoclonal Antibody
Entrez基因编号	NP002096
基因符号	H2A.X H2AFX H2AX
纯化方法	Protein G purified
UniProt编号	P16104

产品使用声明
质量保证	Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected phospho-Histone H2A. X (Ser139) in 200 µg in staurosporine treated HeLa cells.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at 2-8°C from date of receipt.

包装信息
数量	200 µg

merck millipore,默克密理博,05-636-I,Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301

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