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merck millipore,默克密理博,HTS204M,ChemiSCREEN™ Membrane Preparation Recombinant Human Y4 Neuropeptide Y Receptor
产品名称：ChemiSCREEN™ Membrane Preparation Recombinant Human Y4 Neuropeptide Y Receptor
产品型号：HTS204M
Human Y4 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
merck millipore,默克密理博,HTS204M,ChemiSCREEN™ Membrane Preparation Recombinant Human Y4 Neuropeptide Y Receptor
- 产品介绍
- merck millipore,默克密理博,HTS204M,ChemiSCREEN™ Membrane Preparation Recombinant Human Y4 Neuropeptide Y Receptor
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay and GTPγS binding Human Chem-1 Y4 描述 产品目录编号 HTS204M 品牌系列 Chemicon®
商名 - Chemicon
- ChemiScreen
描述 ChemiSCREEN™ Membrane Preparation Recombinant Human Y4 Neuropeptide Y Receptor 概述 Full-length human PPYR1 cDNA encoding Y4 背景信息 The NPY family consists of three 36-amino acid peptides, neuropeptide Y (NPY), peptide YY (PYY) and pancreatic polypeptide (PP), which bind to the NPY receptor family of G protein-coupled receptors. Five NPY receptors, Y1, Y2, Y4, Y5 and Y6, have been defined at the molecular level, and each signals primarily through Gi/o. Binding of NPY family peptides to NPY receptors mediates a variety of physiological effects, including promotion of food intake, decreased anxiety, inhibition of neurotransmitter and hormone release, vasoconstriction, and gut motility. Y4 binds preferentially to PP, with significant binding to NPY and PYY (Michel et al., 1998). Y4 plays a role in control of reproduction, as Y4-null, ob/ob double knockout mice have increased fertility relative to ob/ob mice with functional Y4 (Sainsbury et al., 2002). In addition, mice lacking Y4 display resistance to weight gain induced by high fat diet, and mice lacking both Y2 and Y4 are resistant to the enhanced weight gain exhibited by Y1-null mice fed a high fat diet (Sainsbury et al., 2006). Millipore's Y4 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists of Y4. The membrane preparations exhibit a Kd of 0.35 nM for [125I]-Pancreatic Polypeptide. With 0.4 nM [125I]-Pancreatic Polypeptide, 5μg/well Y4 Membrane Prep typically yields greater than 20-fold signal-to-background ratio. 产品信息 演示 Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membranes protein were adjusted to the indicated concentration in 1 ml packaging buffer, rapidly frozen, and stored at -80°C.应用 应用 Human Y4 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding. 应用说明 Radioligand binding assay and GTPγS binding 生物信息 品种 Human Entrez基因编号 - NM_005972
GPCR类别 A 宿主细胞 Chem-1 蛋白靶 Y4 靶子系列 Neuropeptide Y 孵化条件 Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1mM CaCl2, 0.2% BSA filtered and stored at 4°C
Radioligand: [125I]-Pancreatic Polypeptide. (Perkin Elmer #: NEX315 )
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl, 0.1% BSA filtered and stored at 4°C.
One package contains enough membranes for at least 200 assays (units), where a unit is the amount of membrane that will yield greater than 20-fold signal:background with 125I labeled Pancreatic Polypeptide at 0.4 nM.产品使用声明 质量保证 Table 1. Signal:background and specific binding values obtained in a competition binding assay with 5 μg/well of Y4 Receptor membrane prep. 5 µg/well Signal:Background 37.5 Specific Binding (cpm) 9558
SPECIFICATIONS: 1 unit = 5 µg
Bmax for [125I]-Pancreatic Polypeptide binding: 3.7 pmol/mg protein
Kd for [125I]-Pancreatic Polypeptide binding: ~0.35 nM使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. 包装信息 数量 200 units
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