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merck millipore,默克密理博,HTS182M,ChemiSCREEN™ Membrane Preparation Recombinant Human PK2 Prokineticin Receptor
产品名称：ChemiSCREEN™ Membrane Preparation Recombinant Human PK2 Prokineticin Receptor
产品型号：HTS182M
Human PK2 / PKR2 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
merck millipore,默克密理博,HTS182M,ChemiSCREEN™ Membrane Preparation Recombinant Human PK2 Prokineticin Receptor
- 产品介绍
- merck millipore,默克密理博,HTS182M,ChemiSCREEN™ Membrane Preparation Recombinant Human PK2 Prokineticin Receptor
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay and GTPγS binding. Human Chem-1 PK2 / PKR2 描述 产品目录编号 HTS182M 商名 - Chemicon
- ChemiScreen
描述 ChemiSCREEN™ Membrane Preparation Recombinant Human PK2 Prokineticin Receptor 概述 Full-length human GPR73L1 cDNA encoding PK2 Alternate Names - EG-VEGF
背景信息 Prokineticins, also known as endocrine gland vascular endothelial growth factors (EG-VEGF), are two ~10 kD secreted proteins originally described to mediate angiogenesis and gastrointestinal smooth muscle contraction (Li et al., 2001; LeCouter et al., 2003). Subsequently, prokineticins have been found to mediate central nervous system functions including circadian rhythms and olfactory bulb development (Cheng et al., 2002; Ng et al., 2005). Two Gq-coupled receptors, PK1 and PK2 (also known as GPR73a and GPR73b), mediate cellular responses to prokineticins (Lin et al., 2002). Millipore's PK2 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists at PK2. The membrane preparations exhibit a Kd of 0.36 nM for [125I]-MIT-1. With 0.3 nM [125I]-MIT-1, 10µg/well PK2 Membrane Prep typically yields greater than 5-fold signal-to-background ratio. 产品信息 格式 Membranes 演示 One package contains enough membranes for at least 200 assays (units), where a unit is the amount of membrane that will yield greater than 5-fold signal:background with 125I-labeled MIT-1 at 0.3 nM
Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membranes protein were adjusted to 1 mg/mL in packaging buffer, and dispensed at 1 mL/vial. Vials were rapidly frozen, and stored at -80°C.应用 应用 Human PK2 / PKR2 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding. 应用说明 Radioligand binding assay and GTPγS binding. 生物信息 品种 Human Entrez基因编号 - NM_144773.2
Entrez基因汇总 Prokineticins are secreted proteins that can promote angiogenesis and induce strong gastrointestinal smooth muscle contraction. The protein encoded by this gene is an integral membrane protein and G protein-coupled receptor for prokineticins. The encoded protein is similar in sequence to GPR73, another G protein-coupled receptor for prokineticins. 基因符号 - GPRg2
- PROKR2
- PKR2
- GPR73L1
- GPR73b
- PK-R2
- dJ680N4.3
- OTTHUMP00000030192
- KAL3
GPCR类别 A 宿主细胞 Chem-1 蛋白靶 PK2 / PKR2 靶子系列 Prokineticin UniProt编号 - Q8NFJ6
UniProt汇总 FUNCTION: SwissProt: Q8NFJ6 # Receptor for prokineticin 2. Exclusively coupled to the G(q) subclass of heteromeric G proteins. Activation leads to mobilization of calcium, stimulation of phosphoinositide turnover and activation of p44/p42 mitogen-activated protein kinase.
SIZE: 384 amino acids; 43996 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in the ileocecum, thyroid gland, pituitary gland, salivary gland, adrenal gland, testis, ovary and brain.
DISEASE: SwissProt: Q8NFJ6 # Defects in PROKR2 are the cause of Kallmann syndrome type 3 (KAL3) [MIM:244200]; also known as hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin- releasing hormone-synthesizing neurons. KAL3 patients have variable degrees of olfactory and reproductive dysfunction, but do not show any of the occasional clinical anomalies reported in Kallmann syndrome such as renal agenesis, cleft lip and/or palate, selective tooth agenesis, and bimanual synkinesis.
SIMILARITY: SwissProt: Q8NFJ6 ## Belongs to the G-protein coupled receptor 1 family.孵化条件 Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
Radioligand: [125I] MIT-1 (Perkin Elmer#: NEX-410)
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.产品使用声明 质量保证
SPECIFICATIONS: 1 unit = 10 µg10 µg/well Signal:Background 9.80 Specific Binding (cpm) 6874
Bmax for [125I]- MIT-1 binding: 0.52 pmol/mg protein
Kd for [125I]- MIT-1 binding: ~0.36 nM使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. 包装信息 数量 200 units
上一件merck millipore产品：merck millipore,默克密理博,20-176,100X GTPγS, 10mM
下一件merck millipore产品：merck millipore,默克密理博,345836,Genistin - CAS 529-59-9 - Calbiochem
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