merck millipore,默克密理博,HTS174RTA,Ready-to-Assay™ Secretin Glucagon Receptor Frozen Cells

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merck millipore,默克密理博,HTS174RTA,Ready-to-Assay™ Secretin Glucagon Receptor Frozen Cells
产品名称：Ready-to-Assay™ Secretin Glucagon Receptor Frozen Cells
产品型号：HTS174RTA
Human secretin receptor / SEC GPCR frozen cells for Calcium Flux FLIPR Assays & cAMP Assays.

merck millipore,默克密理博,HTS174RTA,Ready-to-Assay™ Secretin Glucagon Receptor Frozen Cells

产品介绍

merck millipore,默克密理博,HTS174RTA,Ready-to-Assay™ Secretin Glucagon Receptor Frozen Cells

重要规格表

应用说明	品种	宿主细胞	蛋白靶
Calcium Flux Assays; cAMP Assays	Human	Chem-1	secretin receptor / SEC

描述
产品目录编号	HTS174RTA
商名	Ready-to-Assay
描述	Ready-to-Assay™ Secretin Glucagon Receptor Frozen Cells
背景信息	Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response. Secretin, a member of the secretin-glucagon peptide hormone family, is a 27 amino acid peptide that was originally isolated from the duodenum. In the duodenum, secretin is released to stimulate the release of digestive juices in the pancreas (Bayliss et al, 1902). The receptor for secretin is a class 2 (or class B) G protein coupled receptor that signals through Gs to stimulate cAMP production (Dong et al, 2002). Along with its traditional role in the pancreas, studies in secretin-deficient mice have shown miscommunication between the CA3 Schaffer collateral and CA1 pyramidal neurons, causing a deficiency in synaptic transmission (Nishijima et al, 2006). This miscommunication of CA1 dendrites is found in Autism, Rett Syndrome, and most forms of mental retardation, suggesting secretin could be a potential target for treatment of these disorders. Millipore’s cloned human Secretin-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant Secretin expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at Secretin.

产品信息
部件	Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
检测方法	Fluorescent

应用
应用	Human secretin receptor / SEC GPCR frozen cells for Calcium Flux FLIPR Assays & cAMP Assays.
应用说明	Calcium Flux Assays; cAMP Assays

生物信息
品种	Human
Entrez基因编号	NM_002980
GPCR类别	B
宿主细胞	Chem-1
蛋白靶	secretin receptor / SEC
靶子系列	Glucagon
细胞系类型	Ready-to-Assay

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.

产品使用声明

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.