merck millipore,默克密理博,HTS173RTA,Ready-to-Assay™ PTH2 Parathyroid Hormone Receptor Frozen Cells

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merck millipore,默克密理博,HTS173RTA,Ready-to-Assay™ PTH2 Parathyroid Hormone Receptor Frozen Cells
产品名称：Ready-to-Assay™ PTH2 Parathyroid Hormone Receptor Frozen Cells
产品型号：HTS173RTA
Human PTH2 GPCR frozen cells for Calcium Flux FLIPR Assays & cAMP Assays.

merck millipore,默克密理博,HTS173RTA,Ready-to-Assay™ PTH2 Parathyroid Hormone Receptor Frozen Cells

产品介绍

merck millipore,默克密理博,HTS173RTA,Ready-to-Assay™ PTH2 Parathyroid Hormone Receptor Frozen Cells

重要规格表

应用说明	品种	宿主细胞	蛋白靶
Calcium Flux Assays; cAMP Assays	Human	Chem-1	PTH2

描述
产品目录编号	HTS173RTA
商名	Ready-to-Assay
描述	Ready-to-Assay™ PTH2 Parathyroid Hormone Receptor Frozen Cells
背景信息	Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response. Parathyroid hormone (PTH) is recognized by three different class B GPCRs; PTH1, PTH2, and PTH3, which couple to Gs to stimulate cAMP production. The PTH1 receptor is found in high levels in the kidney and bone, where it binds PTH and PTHrP to regulate Ca2+ homeostasis (Brown et al, 1996). The PTH2 receptor, which binds PTH and the neuropeptide tuberoinfundibular peptide 39 (TIP-39) but not PTHrP, has about 50% amino acid sequence identity to the PTH1 receptor. PTH2 is found in the greatest volume in the nervous system and at a very low density in the kidney and bone (Usdin et al, 1995). The PTH3 receptor, which has been recently discovered in the zebrafish, has 61% amino acid identity to the zebrafish PTH1 receptor and also seems to share its ligand affinity (Rubin et al, 1999). Studies with the selective PTH2 receptor agonist, TIP-39, and the distribution of the receptor in the superficial dorsal horn of the spinal cord suggest the receptor may play a role in pain perception (Usdin et al, 1999). Millipore’s cloned human PTH2-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant PTH2 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at PTH2.

产品信息
部件	Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
检测方法	Fluorescent

应用
应用	Human PTH2 GPCR frozen cells for Calcium Flux FLIPR Assays & cAMP Assays.
应用说明	Calcium Flux Assays; cAMP Assays

生物信息
品种	Human
Entrez基因编号	NM_005048
GPCR类别	B
宿主细胞	Chem-1
蛋白靶	PTH2
靶子系列	PTH receptor
细胞系类型	Ready-to-Assay

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.

产品使用声明

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.