merck millipore,默克密理博,HTS111M,ChemiSCREEN™ Membrane Preparation Recombinant Human 5-HT6 Serotonin Receptor

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Merck Millipore 默克密理愽

应用说明	品种	宿主细胞	蛋白靶
Radioligand binding assay and GTPγS binding.	Human	Chem-1	5-HT6

描述
产品目录编号	HTS111M
商名	Chemicon ChemiScreen
描述	ChemiSCREEN™ Membrane Preparation Recombinant Human 5-HT₆ Serotonin Receptor
概述	Full-length human HTR6 cDNA encoding the 5-HT₆ Serotonin Receptor
背景信息	The neurotransmitter serotonin/5-hydroxytryptamine (5-HT) regulates a wide variety of neurological functions. A family of 13 receptors (12 GPCRs and one ion channel) mediate the effects of serotonin. The serotonin receptor 5-HT₆ is a Gs coupled receptor expressed solely in the CNS, primarily in the limbic and cortical regions. 5-HT₆ appears to play a role in memory and learning, obesity, psychosis, anxiety and epilepsy (Woolley et al., 2004; Fisas et al., 2006). In particular, a 5-HT₆-selective agonist caused significant weight loss in a rat model of diet-induced obesity. Millipore's 5-HT₆ membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists of 5-HT₆. The membrane preparations exhibit a Kd of 0.44 nM for [¹²⁵I]-SB258585. With 0.25nM [¹²⁵I]-SB258585, 5 μg/well 5-HT₆ Membrane Prep typically yields greater than 6-fold signal-to-background ratio.

产品信息
格式	Membranes
演示	Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives. Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.

应用
应用	Human 5-HT6 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
应用说明	Radioligand binding assay and GTPγS binding.

生物信息
品种	Human
Entrez基因编号	NM_000871
GPCR类别	A
宿主细胞	Chem-1
蛋白靶	5-HT6
靶子系列	Serotonin
孵化条件	RECOMMENDED ASSAY CONDITIONS: Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with the binding buffer. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted. Binding buffer: 20 mM HEPES, 3 mM MgCl₂, 2 mM Ascorbic acid, pH 7.4, filtered and stored at 4°C Radioligand: [¹²⁵I]-SB258585 (Perkin Elmer #:NEX424) Wash Buffer: same as the binding buffer. One package contains enough membranes for at least 200 assays (units), where a unit is the amount of membrane that will yield greater than 6-fold signal:background with ¹²⁵I labeled SB258585 at 0.25 nM

产品使用声明

质量保证

Table 1. Signal:background and specific binding values obtained in a competition binding assay with varying amounts of 5-HT₆ Receptor membrane prep.

	5 µg/well	10 µg/well
Signal:Background	12.8	12.3
Specific Binding (cpm)	10192	12675

SPECIFICATIONS: 1 unit = 5 µg membrane preparation
B_max for [¹²⁵I]-SB258585 binding: 6.1 pmol/mg protein
K_d for [¹²⁵I]-SB258585 binding: ~0.44 nM

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain frozen at –70°C up to expiration date indicated on the label. Do not freeze and thaw.

包装信息
数量	200 units

merck millipore,默克密理博,HTS111M,ChemiSCREEN™ Membrane Preparation Recombinant Human 5-HT6 Serotonin Receptor

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