merck millipore,默克密理博,HTS092LT,ChemiBrite™ EP3 Prostanoid Receptor Frozen Cells

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merck millipore,默克密理博,HTS092LT,ChemiBrite™ EP3 Prostanoid Receptor Frozen Cells
产品名称：ChemiBrite™ EP3 Prostanoid Receptor Frozen Cells
产品型号：HTS092LT
Human EP3 GPCR frozen cells for Calcium Flux FLIPR Assays.

merck millipore,默克密理博,HTS092LT,ChemiBrite™ EP3 Prostanoid Receptor Frozen Cells

产品介绍

merck millipore,默克密理博,HTS092LT,ChemiBrite™ EP3 Prostanoid Receptor Frozen Cells

重要规格表

应用说明	品种	宿主细胞	蛋白靶
Calcium Flux Assays	Human	HEKT	EP3

描述
产品目录编号	HTS092LT
商名	ChemiBrite
描述	ChemiBrite™ EP₃ Prostanoid Receptor Frozen Cells
背景信息	Millipore’s ChemiBrite cells co-express a GPCR along with a novel variant of clytin, a calcium-activated photoprotein, to enable sensitive luminescent detection of ligand-induced calcium flux. The ChemiBrite version of clytin contains a mutation that increases its affinity for calcium to a level that permits detection of cytosolic calcium in many cells with greater sensitivity than other mitochondrially expressed photoproteins. Luminescent calcium assays offer several advantages over fluorescent calcium assays including; lower substrate cost, increased sensitivity, and lack of interference from fluorescent compounds. Like Millipore’s Ready-to-Assay frozen cells, ChemiBrite frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response. Prostanoids are a series of arachidonic acid metabolites produced by the action of cyclooxygenase and subsequently by isomerases and synthases. Cells rapidly secrete prostanoids after synthesis, whereupon the prostanoids bind to a family of 8 GPCRs to exert their biological effects (Narumiya and FitzGerald, 2001). The prostaglandin PGE2 causes pain, vasodilatation, immunosuppression of T cells, bone resorption and promotion of carcinogenesis. Four related GPCRs, EP1, EP2, EP3 and EP4, each bind to PGE2, but the different G protein coupling status of each receptor leads to distinct biological effects. Further diversity is generated by alternative splicing; the human gene for EP3 generates 9 alternatively spliced mRNAs encoding 8 isoforms of EP3 (Kotani et al., 1997). These isoforms of EP3 vary in sequence at their C-termini, and differ in their ability to couple to Gs, Gq or Gi (Kotani et al., 1995). EP3 is required for fever induced by pyrogens, a response long attributed to prostaglandins by the antipyretic action of aspirin and other COX inhibitors (Ushikubi et al., 1998). In animal models of allergy, PGE2-mediated activation of EP3 inhibits inflammation to counteract the allergy-promoting activity of PGD2 (Kunikata et al., 2005). ChemiBrite cells are made by transient transfection of HEK293T cells with optimized quantities of plasmids encoding the receptor and clytin for calcium signaling. Upon thaw, recovery, and loading, the cells are ready for luminescent analysis of agonists, antagonists and modulators at the EP3 receptor.

产品信息
部件	Vial contains 1 ml of mycoplasma-free cells
检测方法	Chemiluminescence

应用
应用	Human EP3 GPCR frozen cells for Calcium Flux FLIPR Assays.
应用说明	Calcium Flux Assays

生物信息
品种	Human
Entrez基因编号	NM_198716
GPCR类别	A
宿主细胞	HEKT
蛋白靶	EP3
靶子系列	Prostanoid

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.

产品使用声明

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.