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品种反应性 主要应用 宿主 格式 抗体类型 R, M, H WB M Purified Monoclonal Antibody 描述 产品目录编号 05-1051 Replaces 04-1149; MABT66 品牌系列 Upstate
商名 - Upstate
描述 Anti-ILK Antibody, clone 6G6.3 Alternate Names - p59ILK
- Integrin-Linked Kinase
- p59
- DKFZp686F1765
背景信息 ILK (Integrin-Linked Kinase) is an intraceullar Ser/Thr kinase with four Ankyrin-like repeats. It is known to play pivotal roles in extracellular matrix mediated signaling. ILK is known to associate with β-Integrins in addition to many other focal adhesion associated proteins. In this context, ILK is involved cell adhesion/migration. The kinase activity appears to be reduced following Integrin activation, and overexpression of p59ILK inhibits cellular adhesion to Integrin substrates. Additionally, ILK has been associated with epithelial mesechymal transition (EMT) in a TGF-β1 induced Smad dependent manner (Li, 2003). Forced expression of ILK also induced the expression of MMP-2 (matrix metalloprotease 2) and promoted cell migration and invasion (Li, 2003). 产品信息 格式 Purified 演示 Protein G purified mouse monoclonal in storage buffer containing 0.1M Tris-Glycine, pH7.4, 15 mM NaCl, and 0.05% NaN3. 应用 应用 Anti-ILK Antibody, clone 6G6.3 detects level of ILK & has been published & validated for use in WB. 主要应用 - Western Blotting
应用说明 Not recommended for IP 生物信息 免疫原品种 Full length recombinant GST-tagged human ILK. 克隆 6G6.3 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Recognizes ILK 同种型 IgG3κ 品种反应性 RatMouseHuman 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_004508.1
Entrez基因汇总 Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq] 基因符号 - DKFZp686F1765
- ILK
- p59ILK
- ILK-1
- P59
- ILK1
- ILK-2
- ILK2
纯化方法 Protein G purfied UniProt编号 - Q13418
UniProt汇总 FUNCTION: SwissProt: Q13418 # Receptor-proximal protein kinase regulating integrin- mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
SIZE: 452 amino acids; 51419 Da
SUBUNIT: Interacts with cytoplasmic domain of beta 1 subunit of integrin. Could also interacts with beta 2, beta 3 and/or beta 5 subunit of integrin. Interacts with LIMS1, LIMS2, parvins and probably TGFB1I1.
SUBCELLULAR LOCATION: Cell junction, focal adhesion; Cytoplasmic side.
TISSUE SPECIFICITY: Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.DOMAIN:SwissProt: Q13418 A PH-like domain is involved in phosphatidylinositol phosphate binding.
PTM: Autophosphorylated on serine residues.
SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. & Contains 3 ANK repeats. & Contains 1 protein kinase domain.产品使用声明 质量保证 Routinely evaluated by western blot on HEK293 cell lysate. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at 2-8°C.包装信息 数量 100 µg
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