- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,HTS091M,ChemiSCREEN™ Human DP Prostanoid Receptor Membrane Preparation
产品名称：ChemiSCREEN™ Human DP Prostanoid Receptor Membrane Preparation
产品型号：HTS091M
Human DP GPCR membrane preparation for Radioligand binding Assays & GTPgammaS binding.
merck millipore,默克密理博,HTS091M,ChemiSCREEN™ Human DP Prostanoid Receptor Membrane Preparation
- 产品介绍
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay and GTPS binding. Human Chem-1 DP 描述 产品目录编号 HTS091M 品牌系列 Chemicon®
商名 - Chemicon
- ChemiScreen
描述 ChemiSCREEN™ Human DP Prostanoid Receptor Membrane Preparation 概述 Full-length human PTGDR cDNA encoding DP 背景信息 Prostanoids are a series of arachidonic acid metabolites produced by the action of cyclooxygenase and subsequently by isomerases and synthases. Cells rapidly secrete prostanoids after synthesis, whereupon the prostanoids bind to a family of 8 GPCRs to exert their biological effects (Narumiya and FitzGerald, 2001). The prostaglandin PGD2 is produced by mast cells upon activation by allergens, and is present at high levels in allergic diseases. PGD2 binds to two receptors, DP and CRTH2. DP activates Gs to increase cAMP levels, and lack of DP results in reduced allergic response in animal models of bronchial asthma (Matsuoka et al., 2000). Chemicon's DP membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of DP interactions and its ligands. The membrane preparations exhibit a Kd of 10.39 nM for [3H]-PGD2. With 8 nM [3H]-PGD2, 5 µg/well and 10 µg/well DP Membrane Prep typically yield greater than 4-fold signal-to-background ratio. 产品信息 演示 Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA no preservatives.
Packaging method: Membranes protein were adjusted to 0.5 mg/ml in 1 ml packaging buffer, rapidly frozen, and stored at -80°C.应用 应用 Human DP GPCR membrane preparation for Radioligand binding Assays & GTPgammaS binding. 应用说明 Radioligand binding assay and GTPS binding. 生物信息 品种 Human Entrez基因编号 - NM_000953.2
Entrez基因汇总 The protein encoded by this gene is a G-protein-coupled receptor. It has been shown to function as a prostanoid DP receptor. The activity of this receptor is mainly mediated by G-S proteins that stimulate adenylate cyclase resulting in an elevation of intracellular cAMP and Ca2+. Knockout studies in mice suggest that the ligand of this receptor, prostaglandin D2 (PGD2), functions as a mast cell-derived mediator to trigger asthmatic responses. 基因符号 - ASRT1
- PTGDR
- DP
- MGC49004
- AS1
GPCR类别 A 宿主细胞 Chem-1 蛋白靶 DP 靶子系列 Prostanoid UniProt编号 - Q13258
UniProt汇总 FUNCTION: SwissProt: Q13258 # Receptor for prostaglandin D2 (PGD2). The activity of this receptor is mainly mediated by G(s) proteins that stimulate adenylate cyclase, resulting in an elevation of intracellular cAMP. A mobilization of calcium is also observed, but without formation of inositol 1,4,5-trisphosphate.
SIZE: 359 amino acids; 40271 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in retina and small intestine.
DISEASE:SwissProt: Q13258 # Genetic variations in PTGDR are associated with susceptibility to asthma-related traits type 1 (ASRT1) [MIM:607277]. Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing and dyspnea.
SIMILARITY: SwissProt: Q13258 ## Belongs to the G-protein coupled receptor 1 family.孵化条件 Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
Radioligand: [3H] PGD2 (Perkin Elmer#:NET-616 )
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.
One package contains enough membranes for at least 200 assays (units), where a unit is the amount of membrane that will yield greater than 4-fold signal:background with 3H-labeled PGD2 at 8 nM产品使用声明 质量保证 Signal:background and specific binding values obtained in a competition binding assay with varying amounts of DP membrane prep:
10 µg/well 5 µg/well Signal:Background 4.86 4.12 Specific Binding (cpm) 1345 885
Table 2. IC50 values for ligands obtained in a competition binding assay with DP membrane preparation.IC50 (nM) Prostaglanding D2 12.1 13,14-dihydro-15-keto prostaglandin D2 >10000 BW A868C 4.8
SPECIFICATIONS: 1 unit = 10 µg
Bmax for [3H] PGD2 binding: 5.3 pmol/mg protein;
Kd for [3H] PGD2 binding: ~10.39nM使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. 包装信息 数量 200 units
上一件merck millipore产品：merck millipore,默克密理博,435300,LFM-A13 - CAS 62004-35-7 - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,70978,BetaRed™ β-Galactosidase Assay Kit
相关产品
merck millipore,默克密理博,ABT160,Anti-Obscurin Antibody
merck millipore,默克密理博,MAB3514,Anti-Heat Shock Protein 60 Antibody, a.a. 383-447, clone LK1
merck millipore,默克密理博,SF-1470,Siglec-4a; Mouse, Cyanine 5 RNA Detection Probe,SmartFlare
merck millipore,默克密理博,AB2960,Anti-UVRAG Antibody
merck millipore,默克密理博,435300,LFM-A13 - CAS 62004-35-7 - Calbiochem
merck millipore,默克密理博,70978,BetaRed™ β-Galactosidase Assay Kit
merck millipore,默克密理博,127100,Allopregnanolone - CAS 516-54-1 - Calbiochem
merck millipore,默克密理博,SF-1631,PGK1; Human, Cyanine 5 RNA Detection Probe,SmartFlare
merck millipore,默克密理博,371955,H-7, Dihydrochloride - CAS 108930-17-2 - Calbiochem