merck millipore,默克密理博,HTS078LT,ChemiBrite™ S1P2 Lysophospholipid Family Receptor Frozen Cells

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merck millipore,默克密理博,HTS078LT,ChemiBrite™ S1P2 Lysophospholipid Family Receptor Frozen Cells
产品名称：ChemiBrite™ S1P2 Lysophospholipid Family Receptor Frozen Cells
产品型号：HTS078LT
Human S1P2 / EDG5 GPCR frozen cells for Calcium Flux FLIPR Assays.

merck millipore,默克密理博,HTS078LT,ChemiBrite™ S1P2 Lysophospholipid Family Receptor Frozen Cells

产品介绍

merck millipore,默克密理博,HTS078LT,ChemiBrite™ S1P2 Lysophospholipid Family Receptor Frozen Cells

重要规格表

应用说明	品种	宿主细胞	蛋白靶
Calcium Flux Assays	Human	HEKT	S1P2 / EDG5

描述
产品目录编号	HTS078LT
商名	ChemiBrite
描述	ChemiBrite™ S₁P₂ Lysophospholipid Family Receptor Frozen Cells
背景信息	Millipore’s ChemiBrite cells co-express a GPCR along with a novel variant of clytin, a calcium-activated photoprotein, to enable sensitive luminescent detection of ligand-induced calcium flux. The ChemiBrite version of clytin contains a mutation that increases its affinity for calcium to a level that permits detection of cytosolic calcium in many cells with greater sensitivity than other mitochondrially expressed photoproteins. Luminescent calcium assays offer several advantages over fluorescent calcium assays including; lower substrate cost, increased sensitivity, and lack of interference from fluorescent compounds. Like Millipore’s Ready-to-Assay frozen cells, ChemiBrite frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response. Sphingosine 1-phosphate (S1P) is a bioactive lipid that binds to and activates a family of GPCRs, S1P1-5 (also known as EDG receptors). Interactions between S1P and its receptors mediate cytoskeletal rearrangement and cell migration, with functional consequences in angiogenesis, lymphocyte trafficking, and smooth muscle development (Anliker and Chun, 2004). S1P1 (Edg-1) signals exclusively through Gi, whereas S1P2 (Edg-5) and S1P3 (Edg-3) activate Gi, Gq and G12/13 (Windh et al., 1999). Although S1P1 and S1P3 promote cell migration, S1P2 inhibits cell migration in several cell types; these opposing functions appear to result from differences in the ability of each receptor to activate Gi (Arikawa et al., 2003; Sugimoto et al., 2003; Goparaju et al., 2005). Studies with knockout mice indicate that S1P2 and S1P3 have redundant functions in maintaining vascular integrity during embryonic development (Kono et al., 2004). Millipore’s cloned S1P2 receptor-expressing ChemiBrite cells are made by transient transfection of HEK293T cells of optimized quantities of plasmids encoding the receptor and clytin, then cryopreserving the cells at an optimal time post-transfection. Upon thaw, recovery, and loading, the cells are ready for luminescent analysis of agonists, antagonists and modulators at the SIP2 receptor.

产品信息
部件	Vial contains 1 ml of mycoplasma-free cells
检测方法	Chemiluminescence

应用
应用	Human S1P2 / EDG5 GPCR frozen cells for Calcium Flux FLIPR Assays.
应用说明	Calcium Flux Assays

生物信息
品种	Human
Entrez基因编号	NM_004230
GPCR类别	A
宿主细胞	HEKT
蛋白靶	S1P2 / EDG5
靶子系列	Lysophospholipid

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.

产品使用声明

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.