merck millipore,默克密理博,ABE435-S,Anti-trimethyl-Histone H3 (Lys36) Antibody, Trial Size

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Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
M, H	ChIP, WB, ICC	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	ABE435-S
描述	Anti-trimethyl-Histone H3 (Lys36) Antibody, Trial Size
Alternate Names	H3K36me3 Histone H3.1 Histone H3/a Histone H3/b Histone H3/c Histone H3/d Histone H3/f Histone H3/h Histone H3/I Histone H3/j Histone H3/k Histone H3/l
背景信息	Histones are nuclear proteins that form octameric structures which bind DNA to form units of chromatin called nucleosomes. The family of histones—H2A, H2B, H3, and H4—are key players in gene regulation. They undergo a number of post-translational modifications (PTM) in response to various stimuli including phosphorylation on serine and threonine residues and methylation on lysine residues. PTMs produce configural changes in histone proteins that may induce nucleosome remodeling and expose or hide DNA sequences from transcriptional complexes. Methylation occurs on histone H3 at lysine 4, 9, 27, 36, and 79 residues. Previous studies have reported that methylation of H3 lysine 9 provides a binding site for heterochromatin adaptor proteins (HP1) which are involved in the formation of heterochromatin and gene repression. Phosphorylation of histones may also produce similar repressive effects depending on the cellular context. Often, it is the pattern of different modifications on several histone residues that alter chromatin, rather than modification of single residues.

产品信息
格式	Affinity Purified
控制	HeLa cell extract and recombinant Histone H3 protein
演示	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

应用
应用	Anti-trimethyl-Histone H3 (Lys36) Antibody, Trial Size, is a rabbit polyclonal antibody for detection of Histone H3 trimethylated at lysine 36 (H3K36me3). This purified Ab is specificity verified by dot blot (DB) and validated in WB, ICC. Small pack size is ideal for screening applications.
主要应用	Chromatin Immunoprecipitation (ChIP) Western Blotting Immunocytochemistry
应用说明	Dot Blot Specificity Analysis: Unmodified and various modified Histone peptides (see Table) were probed with Anti-trimethyl-Histone H3 (Lys36) (1:2,000 dilution). Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Histone H3 in A431 cells. ChIP-Sequencing: Chromatin immunoprecipitation was performed using the Magna ChIP™ HiSens kit (cat#17-10460, 2 µg of anti-trimethyl-Histone H3 (Lys36) antibody(ABE435-S), 20 µL Protein A/G beads , and 1e6 crosslinked HeLa cell chromatin followed by DNA purification using magnetic beads. Libraries were prepared from Input and ChIP DNA samples using standard protocols with Illumina barcoded adapters, and analyzed on Illumina HiSeq™ instrument. An excess of eleven million reads from FastQ files were mapped using Bowtie following TagDust tag removal. Peaks were identified using MACS , with peaks and reads visualized as a custom track in UCSC Genome Browser from BigWig and BED files.

生物信息
免疫原品种	KLH-conjugated linear peptide corresponding to human Histone H3 trimethylated at Lys36.
表位	Trimethylated Lys36
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	This antibody recognizes Histone H3 trimethylated at Lys36.
品种反应性	MouseHuman
Species Reactivity Note	Demonstrated to react with Human and Mouse. Broad species reactivity expected due to similar sequence homology.
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_003484
基因符号	MGC126886 HIST1H3A H3FA HIST1H3B H3FL HIST1H3C H3FC HIST1H3D H3FB HIST1H3E H3FD HIST1H3F H3FI HIST1H3G H3FH HIST1H3H H3FK HIST1H3I H3FF HIST1H3J H3FJ HIST3H3 H3FT H3T
纯化方法	Affinity purified
UniProt编号	Q16695

产品使用声明
质量保证	Evaluated by Western Blot in HeLa cell extract and recombinant Histone H3 protein. Western Blot Analysis: A 1:500 dilution of this antibody detected Histone H3 on 10 µg of HeLa cell extract and recombinant Histone H3 protein.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at 2-8°C from date of receipt.

包装信息
数量	20 µL

merck millipore,默克密理博,ABE435-S,Anti-trimethyl-Histone H3 (Lys36) Antibody, Trial Size

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