merck millipore,默克密理博,HTS059M,ChemiScreen™ V1A Vasopressin Membrane Preparation

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Merck Millipore 默克密理愽

应用说明	品种	宿主细胞	蛋白靶
Radioligand binding assay, and GTPγS binding.	Human	Chem-1	V1A

描述
产品目录编号	HTS059M
品牌系列	Chemicon®
商名	ChemiScreen Chemicon
描述	ChemiScreen™ V1A Vasopressin Membrane Preparation
概述	Human AVPR1 cDNA encoding V_1A
背景信息	Arginine vasopressin (AVP) is a 9 amino acid peptide that functions as an antidiuretic, vasoconstrictor and neurotransmitter. The three vasopressin receptors, V_1A, V_1B and V₂, are GPCRs; V_1A and V_1B couple to G_q and calcium release, whereas V₂ couples to G_s. V₁ receptors regulate fluid homeostasis by stimulating contraction of glomerular mesangial cells and renal vascular smooth muscle cells (Birnbaumer, 2000). Chemicon's V_1A membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of V_1A interactions with its ligands. The membrane preparations exhibit a Kd of 55 pM for [¹²⁵I]-vasopressin antagonist ([¹²⁵I]-phenylacetyl-D-Tyr(Me)-Phe-Gln-Asn-Arg -Pro-Arg-Tyr-NH2). With 10 µg/well V_1A Membrane Prep and 0.1 nM [¹²⁵]-vasopressin V_1A antagonist, greater than 12-fold signal-to-background ratio were obtained.

产品信息
格式	Cell Line
演示	Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA no preservatives. Packaging method: Membranes protein was adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.

应用
应用	Human V1A GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
应用说明	Radioligand binding assay, and GTPγS binding.

生物信息
浓缩	0.5 mg/mL
品种	Human
品种反应性	Human
Entrez基因编号	NM_000706.3
Entrez基因汇总	The protein encoded by this gene acts as receptor for arginine vasopressin. This receptor belongs to the subfamily of G-protein coupled receptors which includes AVPR1B, V2R and OXT receptors. Its activity is mediated by G proteins which stimulate a phosphatidylinositol-calcium second messenger system. The receptor mediates cell contraction and proliferation, platelet aggregation, release of coagulation factor and glycogenolysis.
基因符号	V1aR AVPR1A AVPR1
GPCR类别	A
宿主细胞	Chem-1
蛋白靶	V1A
靶子系列	Vasopressin
UniProt编号	P37288
UniProt汇总	FUNCTION: SwissProt: P37288 # Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate a phosphatidyl- inositol-calcium second messenger system. Has been involved in social behaviors, including affiliation and attachment. SIZE: 418 amino acids; 46800 Da SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. SIMILARITY: SwissProt: P37288 ## Belongs to the G-protein coupled receptor 1 family. MISCELLANEOUS: Differences in regional receptor expression in the brain as well as differences in social behavior may result from a highly variable repetitive sequence in the 5' flanking region of AVPR1A. One such allelic variant has been linked to autism.

产品使用声明

质量保证

Signal:background and specific binding values obtained in a competition binding assay with varying amounts of V_1A membrane prep:

	10 µg/well
Signal:Background	24.4
Specific Binding (cpm)	11827

SPECIFICATIONS:
1 unit = 10 µg
Bmax: 1.76 pmol/mg
Kd: 55 pM

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.