merck millipore,默克密理博,HTS054M,ChemiScreen™ CXCR6 Membrane Preparation

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merck millipore,默克密理博,HTS054M,ChemiScreen™ CXCR6 Membrane Preparation
产品名称：ChemiScreen™ CXCR6 Membrane Preparation
产品型号：HTS054M
Human CXCR6 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.

merck millipore,默克密理博,HTS054M,ChemiScreen™ CXCR6 Membrane Preparation

产品介绍

merck millipore,默克密理博,HTS054M,ChemiScreen™ CXCR6 Membrane Preparation

重要规格表

应用说明	品种	宿主细胞	蛋白靶
Radioligand binding assay, and GTPγS binding.	Human	Chem-5	CXCR6

描述
产品目录编号	HTS054M
品牌系列	Chemicon®
商名	Chemicon ChemiScreen
描述	ChemiScreen™ CXCR6 Membrane Preparation
概述	Human CXCR6 cDNA
Alternate Names	TYMSTR BONZO STRL33
背景信息	The GPCR CXCR6 (previously known as BONZO, STRL33 and TYMSTR) binds selectively to the free chemokine domain of CXCL16, which is derived from a membrane-bound precursor containing a CXC-containing chemokine domain, a glycosylated mucin-like domain, and a transmembrane domain (Wilbanks et al., 2001) CXCR6 is selectively expressed on Th1, Th2 and Tr1 T cell subsets, whereas CXCL16 is expressed on monocytes/macrophages and dendritic cells (Tabata et al., 2005). CXCR6 promotes migration of activated lymphocytes to sites of inflammation in tissues such as liver and synovium (Nanki et al., 2005, Sato et al., 2005). Chemicon's CXCR6 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of CXCR6 interactions with its ligands. The membrane preparations exhibit a Kd of 0.6 nM for [¹²⁵I]-CXCL16. With 2.5 or 10 μg/well CXCR6 Membrane Prep and 0.5 nM [¹²⁵I]-CXCL16, greater than 4-fold and 9-fold signal-to-background ratios are obtained, respectively.

产品信息
格式	Membranes
演示	One vial contains enough membranes for at least 200 assays (units), where one unit is the amount of membrane that will yield greater than 1000 cpm specific CXCL16-stimulated [³⁵S]-GTPγS binding. If radioligand binding assays are desired, it is recommended to double the mass of membrane prep per well to increase signal window.. Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives. Packaging method: Membrane protein was adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80oC.

产品信息

格式

Membranes

演示

One vial contains enough membranes for at least 200 assays (units), where one unit is the amount of membrane that will yield greater than 1000 cpm specific CXCL16-stimulated [³⁵S]-GTPγS binding. If radioligand binding assays are desired, it is recommended to double the mass of membrane prep per well to increase signal window..

Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.

Packaging method: Membrane protein was adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80oC.

应用
应用	Human CXCR6 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
应用说明	Radioligand binding assay, and GTPγS binding.

生物信息
品种	Human
Entrez基因编号	NM_006564.1
基因符号	STRL33 CXCR6 CDw186 BONZO CXC-R6 CXCR-6 TYMSTR CD186
GPCR类别	A
宿主细胞	Chem-5
蛋白靶	CXCR6
靶子系列	Chemokine
UniProt编号	O00574
UniProt汇总	FUNCTION: SwissProt: O00574 # Receptor for the C-X-C chemokine CXCL16. Used as a coreceptor by SIVs and by strains of HIV-2 and m-tropic HIV-1. SIZE: 342 amino acids; 39280 Da SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed in lymphoid tissues and activated T cells. SIMILARITY: SwissProt: O00574 ## Belongs to the G-protein coupled receptor 1 family.
孵化条件	RECOMMENDED ASSAY CONDITIONS: Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted. Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl₂, 1 mM CaCl₂, 0.2% BSA, filtered and stored at 4°C Radioligand: ^[125I] CXCL16 (Perkin Elmer catalog # NEX398) Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C. One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 4-fold signal:background with ¹²⁵I-labeled CXCL16 at 0.5 nM

产品使用声明
质量保证	SPECIFICATIONS: 1 unit = 5 μg for GTPγS binding assay EC50 in GTPγS binding assay by CXCL16: 0.52 – 1.2 nM
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.