merck millipore,默克密理愽
  • merck millipore,默克密理博,HTS015M,ChemiSCREEN™ CX3CR1 Membrane Preparation

  • merck millipore,默克密理博,HTS015M,ChemiSCREEN™ CX3CR1 Membrane Preparation

    产品名称：ChemiSCREEN™ CX3CR1 Membrane Preparation
    产品型号：HTS015M
    Human CX3CR1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.

    merck millipore,默克密理博,HTS015M,ChemiSCREEN™ CX3CR1 Membrane Preparation

  • 产品介绍
  • merck millipore,默克密理博,HTS015M,ChemiSCREEN™ CX3CR1 Membrane Preparation

    重要规格表

    应用说明 品种 宿主细胞 蛋白靶
    Radioligand binding assay, and GTPγS binding. HumanChem-1CX3CR1
    描述
    产品目录编号 HTS015M
    品牌系列 Chemicon®
    商名
    • Chemicon
    • ChemiScreen
    描述 ChemiSCREEN™ CX3CR1 Membrane Preparation
    概述 Full-length Human CX3CR1
    Alternate Names
    • V28
    背景信息 CX3CR1 is a GPCR expressed on natural killer cells, cytotoxic T lymphocytes, and macrophages. The sole ligand for CX3CR1, fractalkine, is an unusual chemokine that is expressed as a transmembrane molecule with a CX3C domain and a mucin domain (Imai et al., 1997). Fractalkine is highly expressed on endothelial cells activated by TNFa and other proinflammatory cytokines, and fractalkine/CX3CR1 interactions mediate recruitment of macrophages into the atherosclerotic plaque (Lesnick et al., 2003; McDermott et al., 2003). In addition, fractalkine and CX3CR1 have been implicated in the pathogenesis of glomerulonephritis, HIV infection and rheumatoid arthritis (Ito et al., 2002; Faure et al., 2003; Nanki et al., 2002). Chemicon's CX3CR1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of fractalkine/CX3CR1 interactions. The membrane preparations exhibit a Kd of 0.05 nM for [125I]-fractalkine. With 2.5 mg/well CX3CR1 Membrane Prep and 0.05 nM [125I]-fractalkine, a greater than 7-fold signal-to-background ratio is obtained.
    产品信息
    格式 Membranes
    演示 Packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA

    Packaging method: Membranes protein were adjusted to 1 mg/ml in 1 ml packaging buffer, rapidly frozen, and stored at -80°C.
    应用
    应用 Human CX3CR1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
    应用说明 Radioligand binding assay, and GTPγS binding.
    生物信息
    浓缩 1 mL, 1 mg/mL
    品种 Human
    品种反应性 Human
    Entrez基因编号
    • NM_001337.3
    基因符号
    • CMKBLR1
    • CX3CR1
    • CMKDR1
    • GPR13
    • CMK-BRL-1
    • V28
    • GPRV28
    • CMKBRL1
    • CCRL1
    GPCR类别 A
    宿主细胞 Chem-1
    蛋白靶 CX3CR1
    靶子系列 Chemokine
    UniProt编号
    • P49238
    UniProt汇总 FUNCTION: SwissProt: P49238 # Receptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV coreceptors than isoform 1.
    SIZE: 355 amino acids; 40396 Da
    SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
    TISSUE SPECIFICITY: Expressed in lymphoid and neural tissues.
    SIMILARITY: SwissProt: P49238 ## Belongs to the G-protein coupled receptor 1 family.
    孵化条件 RECOMMENDED ASSAY CONDITIONS:

    Binding buffer: 50mM HEPES, 5mM MgCl2, 1mM CaCl2, 0.2% BSA, pH to 7.4 and filter.

    Radioligand: [125I] Fractalkine (PerkinElmer#: NEX368)

    One vial contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 5-fold signal:background with 125I-labeled fractalkine at 0.05 nM.
    产品使用声明
    质量保证 Bmax: 3.13 pmol/mg

    Kd: 0.05 nM
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw.
    包装信息
    数量 200 units

    merck millipore,默克密理博,HTS015M,ChemiSCREEN™ CX3CR1 Membrane Preparation

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