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merck millipore,默克密理博,05-1238,Anti-PARN Antibody, clone 7A7.1
产品名称：Anti-PARN Antibody, clone 7A7.1
产品型号：05-1238
Use Anti-PARN Antibody, clone 7A7.1 (Mouse Monoclonal Antibody) validated in WB, ICC to detect PARN also known as Poly(A)-specific ribonuclease PARN, Deadenylating nuclease.
merck millipore,默克密理博,05-1238,Anti-PARN Antibody, clone 7A7.1
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H ICC, WB M Purified Monoclonal Antibody 描述 产品目录编号 05-1238 描述 Anti-PARN Antibody, clone 7A7.1 Alternate Names - Polyadenylate-specific ribonuclease
- Poly(A)-specific ribonuclease PARN
- Deadenylating nuclease
- Deadenylation nuclease
背景信息 Poly(A) ribonuclease (PARN) is a exoribonuclease responsible for the deadenylation of mRNA. This protein is constitutively expressed in virtually all mammalian tissues. With a critical role in the post-transcriptional control of gene expression PARN consists of two RNA-binding domains and one catalytic domain. Although PARN has a preference for poly(A), data suggests the protein can also degrade poly(U). 产品信息 格式 Purified 控制 - A431 cell lysate
演示 Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide. 应用 应用 Use Anti-PARN Antibody, clone 7A7.1 (Mouse Monoclonal Antibody) validated in WB, ICC to detect PARN also known as Poly(A)-specific ribonuclease PARN, Deadenylating nuclease. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected PARN, clone 7A7.1 in HeLa cells. 生物信息 免疫原品种 Linear peptide corresponding to human PARN. 克隆 7A7.1 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 同种型 IgMκ 品种反应性 Human Species Reactivity Note Demonstrated to react with Human. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_002573
Entrez基因汇总 Summary: The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. 基因符号 - DAN
- PARN
纯化方法 Ion exchange UniProt编号 - O95453
UniProt汇总 FUNCTION: 3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization.
CATALYTIC SCTIVITY: Exonucleolytic cleavage of poly(A) to 5'-AMP.
COFACTOR: Divalent metal cations. Mg2+ is the most probable.
SUBUNIT STRUCTURE: Homodimer. Interacts with KHSRP and CELF1/CUGBP1. Found in a mRNA decay complex with RENT1, RENT2 and RENT3B.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Nucleus › nucleolus. Note: Some nuclear fraction is nucleolar.
TISSUE SPECIFICITY: Ubiquitous.
SEQUENCE SIMILARITIES: Belongs to the CAF1 family. Contains 1 R3H domain.产品使用声明 质量保证 Evaluated by Western Blot in A431 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected PARN on 10 µg of A431 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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