merck millipore,默克密理博,HTS014RTA,Ready-to-Assay™ CCR10 Chemokine Family Receptor Frozen Cells

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merck millipore,默克密理博,HTS014RTA,Ready-to-Assay™ CCR10 Chemokine Family Receptor Frozen Cells
产品名称：Ready-to-Assay™ CCR10 Chemokine Family Receptor Frozen Cells
产品型号：HTS014RTA
Human CCR10 GPCR frozen cells for Calcium Flux FLIPR Assays.

merck millipore,默克密理博,HTS014RTA,Ready-to-Assay™ CCR10 Chemokine Family Receptor Frozen Cells

产品介绍

merck millipore,默克密理博,HTS014RTA,Ready-to-Assay™ CCR10 Chemokine Family Receptor Frozen Cells

重要规格表

应用说明	品种	宿主细胞	蛋白靶
Calcium Flux Assays	Human	Chem-1	CCR10

描述
产品目录编号	HTS014RTA
商名	Ready-to-Assay
描述	Ready-to-Assay™ CCR10 Chemokine Family Receptor Frozen Cells
背景信息	Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response. CCR10, previously known as GPR2, is a GPCR that is expressed in dermal endothelial cells, fibroblasts, melanocytes, and circulating T cells and PBMCs (Homey et al., 2000). Two chemokines, CTACK/CCL27 and MEC/CCL28, bind to CCR10 and stimulate migration and calcium flux (Pan et al., 2000; Wang et al., 2000). Expression of CTACK by keratinocytes, and CCR10 by lymphocytes from skin of patients with psoriasis and dermatitis, implicates this receptor ligand pair in skin inflammation. In addition, disruption of CCL27-CCR10 interaction reduces skin inflammation induced by allergen (Homey et al., 2002). CCR10 expressed on IgA antibody-secreting cells also mediates their migration to CCL28 expressed in the lactating mammary gland, and subsequent IgA accumulation in milk (Wilson and Butcher, 2004). Millipore’s cloned human CCR10-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant CCR10 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at CCR10.

产品信息
部件	Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
检测方法	Fluorescent

应用
应用	Human CCR10 GPCR frozen cells for Calcium Flux FLIPR Assays.
应用说明	Calcium Flux Assays

生物信息
品种	Human
Entrez基因编号	AF215981
GPCR类别	A
宿主细胞	Chem-1
蛋白靶	CCR10
靶子系列	Chemokine
细胞系类型	Ready-to-Assay

产品使用声明
使用声明	This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled. Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

使用声明

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.