- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,HTS012M,ChemiSCREEN™ CCR7 Membrane Preparation, recombinant
产品名称：ChemiSCREEN™ CCR7 Membrane Preparation, recombinant
产品型号：HTS012M
Human CCR7 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
merck millipore,默克密理博,HTS012M,ChemiSCREEN™ CCR7 Membrane Preparation, recombinant
- 产品介绍
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay and GTPγS binding Human Chem-2 CCR7 描述 产品目录编号 HTS012M 品牌系列 Chemicon®
商名 - Chemicon
- ChemiScreen
描述 ChemiSCREEN™ CCR7 Membrane Preparation, recombinant 概述 Full-length human CCR7 cDNA 背景信息 CCR7 is a GPCR that binds to CC chemokine ligands MIP-3beta (ELC/CCL19) and 6Ckine (CCL21)1. These ligands are expressed in the secondary lymphoid organs, and binding to CCR7 expressed in naïve T cells, B cells and dendritic cells directs migration of these cells to sites of antigen presentation2. Inhibition of CCR7/ligand interactions inhibits contact sensitivity, delayed type hypersensitivity, and graft vs host disease in experimental models2,3. In addition, CCR7 expression by breast cancer, melanoma and other malignant cells are associated with lymph node metastasis4,5. Chemicon's CCR7 Membrane Preparations are ideal for screening for inhibitors of CCR7/ligand interactions. The CCR7 membrane preparations bind to MIP-3beta with a Kd of approximately 0.2 nM. At a [125I]-MIP-3beta concentration of 0.1 nM, a signal to background ratio of greater than 8-fold is typically obtained. 产品信息 格式 Membranes 演示 Membranes were adjusted to 0.5 mg protein/ml in 50 mM Tris pH 7.4, 10% glycerol and 1% BSA, rapidly frozen, and stored at -80°C, sufficient for 200 microassays per vial. 应用 应用 Human CCR7 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding. 应用说明 Radioligand binding assay and GTPγS binding 生物信息 品种 Human 品种反应性 Human Entrez基因编号 - NM_001838.2
Entrez基因汇总 The protein encoded by this gene is a member of the G protein-coupled receptor family. This receptor was identified as a gene induced by the Epstein-Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. This receptor is expressed in various lymphoid tissues and activates B and T lymphocytes. It has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. The chemokine (C-C motif) ligand 19 (CCL19/ECL) has been reported to be a specific ligand of this receptor. 基因符号 - CC-CKR-7
- CCR7
- CMKBR7
- EBI1
- CD197
- CDw197
- CCR-7
- BLR2
- EVI1
GPCR类别 A 宿主细胞 Chem-2 蛋白靶 CCR7 靶子系列 Chemokine UniProt编号 - P32248
UniProt汇总 FUNCTION: SwissProt: P32248 # Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions.
SIZE: 378 amino acids; 42874 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.
SIMILARITY: SwissProt: P32248 ## Belongs to the G-protein coupled receptor 1 family.孵化条件 Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
Radioligand: [125I] MIP-3β (Perkin Elmer #NEX370 )
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.
One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 7-fold signal:background with [125I]-labeled MIP-3β at 0.1 nM产品使用声明 质量保证 SPECIFICATIONS: 1 unit = 10 µg membrane preparation
Bmax: 3.3 pmol/mg
Kd: 0.2 nM
Table 1. Signal:background and specific binding values obtained in a competition binding assay with CCR7 membrane preparation.10 µg/well Signal:Background 10.2 Specific Binding (cpm) 8362 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. 包装信息 数量 200 assays
上一件merck millipore产品：merck millipore,默克密理博,FCCH100106,FlowCellect MitoDamage Kit
下一件merck millipore产品：merck millipore,默克密理博,480415,NF023 - Calbiochem
相关产品
merck millipore,默克密理博,CC1018,Human Integrin αVβ3 Protein, Triton X-100 Formulation
merck millipore,默克密理博,SF-1081,miR-122-5p; Human, Cyanine 3, RNA Detection Probe,SmartFlare
merck millipore,默克密理博,MABN464,Anti-Cav3.1 Ca2+ channel Antibody, clone N178A/9
merck millipore,默克密理博,AB5360-50UL,Anti-Potassium Channel Kv4.2 Antibody
merck millipore,默克密理博,FCCH100106,FlowCellect MitoDamage Kit
merck millipore,默克密理博,480415,NF023 - Calbiochem
merck millipore,默克密理博,GF25,Anti-VEGF (Ab-3) Mouse mAb (14-124)
merck millipore,默克密理博,MR-020P-5D,EmbryoMax KSOM Embryo Culture (1X), Powder, w/o Phenol Red, 250ml
%5BABC159_IHC(P)-ALL%5D.jpg "merck millipore,默克密理博,ABC159,Anti-MUC-1 Antibody, EC domain")
merck millipore,默克密理博,ABC159,Anti-MUC-1 Antibody, EC domain
merck millipore,默克密理博,EZMTNFA,Mouse TNF-α ELISA Kit, EZMTNFA