merck millipore,默克密理愽
  • merck millipore,默克密理博,FCCH100106,FlowCellect MitoDamage Kit

  • merck millipore,默克密理博,FCCH100106,FlowCellect MitoDamage Kit

    产品名称：FlowCellect MitoDamage Kit
    产品型号：FCCH100106
    This FlowCellectMitoDamage Kit for Flow Cytometry includes MitoSense Red, a fluorescent cationic dye that accumulates in the mitochondria & is responsive to mitochondrial potential changes.

    merck millipore,默克密理博,FCCH100106,FlowCellect MitoDamage Kit

  • 产品介绍
  • merck millipore,默克密理博,FCCH100106,FlowCellect MitoDamage Kit

    描述
    产品目录编号 FCCH100106
    商名
    • FlowCellect
    描述 FlowCellect MitoDamage Kit
    概述 Millipore’s FlowCellect™MitoDamage Kit includes MitoSense Red, a fluorescent cationic dye that accumulates in the mitochondria, and is responsive to mitochondrial potential changes. Annexin V conjugated to a green sensitive dye, CF488A, which binds to phosphatidylserine (PS) on the surface of apoptotic cells. And the cell impermeant DNA intercalator 7-Aminoactinomycin-D (7-AAD), a dead cell dye. The simultaneous use of the reagents allows researchers to obtain information on early, mid and late apoptosis in one simple assay.
    MitoSense Red is excitable by a red laser and fluoresces maximally at 650 nm (Red2 fluorescence on the guava easyCyte 8HT). Uninduced cells with intact mitochondrial membrane potential demonstrate high Red2 fluorescence while cells which have impaired mitochondrial membrane potential depict lower Red2 fluorescence. Annexin V is calcium-dependent phospholipid binding protein with high affinity for PS, a membrane component normally localized to the internal face of the cell membrane. It is conjugated to CF488A which is excited by a 488 nm laser and emits at 525 nm. Control cells depict no green fluorescence while apoptotic cells exhibit positive green fluorescence. The 7-AAD dye included in the kit monitors cell membrane permeability changes typically observed later in apoptosis as well as necrotic cell death. 7-AAD is excluded from live, healthy cells as well as early apoptotic cells and these cells have low red fluorescence. The kit can thus distinguish multiple populations: 1) Live cells with intact mitochondrial membrane 2) Cells with dissipated membrane potential but no Annexin V or 7AAD staining 3) Early apoptotic cells with dissipated membrane potential and Annexin V binding 4) Late Apoptotic Cells or dead cells with dissipated membrane potentials. The kit thereby provides a complete picture of mitochondrial health and cell health and allows the correlation of mitochondrial damage to cell health. The entire assay can be performed in less than30 minutes once cellular samples are ready.
    Alternate Names
    • FlowCellect
    产品信息
    部件
    • User Manual
    • MitoSense Red Dye
    • Annexin CF 488
    • 7-AAD
    • 10X Assay Buffer
    检测方法 Fluorescent
    应用
    应用 This FlowCellectMitoDamage Kit for Flow Cytometry includes MitoSense Red, a fluorescent cationic dye that accumulates in the mitochondria & is responsive to mitochondrial potential changes.
    主要应用
    • Flow Cytometry
    生物信息
    品种反应性 Human
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    包装信息
    数量 100 Tests

    merck millipore,默克密理博,FCCH100106,FlowCellect MitoDamage Kit

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