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merck millipore,默克密理博,HTS001M,ChemiSCREEN™ Membrane Preparation Recombinant Human CXCR1 Chemokine Receptor
产品名称：ChemiSCREEN™ Membrane Preparation Recombinant Human CXCR1 Chemokine Receptor
产品型号：HTS001M
Human CXCR1 / IL-8a GPCR membrane preparation for Radioligand binding Assays & GTPgammaS binding.
merck millipore,默克密理博,HTS001M,ChemiSCREEN™ Membrane Preparation Recombinant Human CXCR1 Chemokine Receptor
- 产品介绍
- merck millipore,默克密理博,HTS001M,ChemiSCREEN™ Membrane Preparation Recombinant Human CXCR1 Chemokine Receptor
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay, and GTPgammaS binding. Human Chem-1 CXCR1 / IL-8a 描述 产品目录编号 HTS001M 品牌系列 Chemicon®
商名 - Chemicon
- ChemiScreen
描述 ChemiSCREEN™ Membrane Preparation Recombinant Human CXCR1 Chemokine Receptor 概述 : Full-length human CXCR1 cDNA 背景信息 CXCR1 (also known as IL-8RA) is a Gi/o-coupled GPCR expressed on neutrophils, CD8(+) T cells, and intestinal epithelial cells (Murphy, 1997; Sturm et al., 2005; Takata et al., 2004). CXCR1 binds specifically to the chemokine interleukin-8 (IL-8), whereas a related receptor, CXCR2, binds promiscuously to IL-8 and several other chemokines. CXCR1 has been proposed to be the primary regulator of neutrophil response to IL-8 in sepsis (Cummings et al., 1999). Antagonists of CXCR1 and CXCR2 have been developed and display efficacy in animal models of reperfusion injury (Bertini, et al., 2004; Kaneider et al., 2005). Millipore's CXCR1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of CXCR1 interaction with IL-8. The membrane preparations exhibit a Kd of 0.2 nM for [125I]-IL-8. With 5 μg/well CXCR1 Membrane Prep and 0.1 nM [125I]-IL-8, a greater than 5-fold signal-to-background ratio is obtained. 产品信息 格式 Membranes 演示 Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA no preservatives.Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C. 应用 应用 Human CXCR1 / IL-8a GPCR membrane preparation for Radioligand binding Assays & GTPgammaS binding. 应用说明 Radioligand binding assay, and GTPgammaS binding. 生物信息 浓缩 1 mg/mL 品种 Human 品种反应性 Human Entrez基因编号 - NM_000634.2
Entrez基因汇总 The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. Knockout studies in mice suggested that this protein inhibits embryonic oligodendrocyte precursor migration in developing spinal cord. This gene, IL8RB, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. 基因符号 - CD181
- IL8RA
- CMKAR1
- CKR-1
- C-C
- C-C-CKR-1
- CXCR-1
- IL8RBA
- IL8R1
- CDw128a
- CD128
- CXCR1
GPCR类别 A 宿主细胞 Chem-1 蛋白靶 CXCR1 / IL-8a 靶子系列 Chemokine UniProt编号 - P25024
UniProt汇总 FUNCTION: SwissProt: P25024 # Receptor to interleukin-8, which is a powerful neutrophils chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activate a phosphatidylinositol-calcium second messenger system. This receptor binds to IL-8 with a high affinity and to MGSA (GRO) with a low affinity.
SIZE: 350 amino acids; 39791 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: P25024 ## Belongs to the G-protein coupled receptor 1 family.孵化条件 RECOMMENDED ASSAY CONDITIONS: Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
Radioligand: [125I] Interleukin-8 (Perkin Elmer #NEX277)
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.
One vial contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 5-fold signal:background with 125I-labeled IL-8 at 0.1 nM产品使用声明 质量保证 Table 1. Signal:background and specific binding values obtained in a competition binding assay with varying amounts of CXCR1 Receptor membrane preparation in a dose response curve with unlabeled IL-8.
5 µg/well 10 µg/well Signal:Background 14.8 12.8 Specific Binding (cpm) 8965 9624
SPECIFICATIONS: 1 unit = 5 μg
Bmax: 3.7 pmol/mg;
Kd: 0.2 nM使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. 包装信息 数量 200 units
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