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merck millipore,默克密理博,HCD8MAG-15K,MILLIPLEX MAP Human CD8+ T Cell Magnetic Bead Panel - Immunology Multiplex Assay
产品名称：MILLIPLEX MAP Human CD8+ T Cell Magnetic Bead Panel - Immunology Multiplex Assay
产品型号：HCD8MAG-15K
The analytes available for this multiplex kit are: GM-CSF, sCD137, IFNγ, sFas, sFasL, Granzyme A, Granzyme B, IL-2, IL-4, IL-5, IL-6, IL-10, IL-13, MIP-1α, MIP-1β, TNF-α, Perforin.
merck millipore,默克密理博,HCD8MAG-15K,MILLIPLEX MAP Human CD8+ T Cell Magnetic Bead Panel - Immunology Multiplex Assay
- 产品介绍
- merck millipore,默克密理博,HCD8MAG-15K,MILLIPLEX MAP Human CD8+ T Cell Magnetic Bead Panel - Immunology Multiplex Assay
重要规格表
可用的分析物 品种反应性 主要应用 检测方法 Perforin, GM-CSF, sCD137, IFNγ, sFas, sFasL, Granzyme A, Granzyme B, IL-2, IL-4, IL-5, IL-6, IL-10, IL-13, MIP-1α, MIP-1β, TNF-α H Mplex Luminex xMAP 描述 产品目录编号 HCD8MAG-15K 商名 - MILLIPLEX
描述 MILLIPLEX MAP Human CD8+ T Cell Magnetic Bead Panel - Immunology Multiplex Assay 概述 The MILLIPLEX MAP Human CD8+ T Cell Magnetic Bead Panel enables you to focus on the therapeutic potential of Human CD8+ T Cell analytes. Coupled with the Luminex® xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of several analytes simultaneously, which can dramatically improve productivity. The MILLIPLEX MAP Human CD8+ T Cell Magnetic Bead Panel is the most versatile system available for Mouse CD8+ T Cell research.
MILLIPLEX MAP offers you the ability to:
• Select a 17-plex premixed option or
• Choose any combination of analytes from our panel of 17 analytes to design a custom kit that better meets your needs.
• A convenient “all-in-one” box format gives you the assurance that you will have all the necessary reagents you need to run your assay.背景信息 CD8+ T cells (also known as cytotoxic T cells, cytolytic T cells and killer T cells) belong to a sub-group of T cells capable of inducing the death of infected somatic or tumor cells. T cells that bind weakly to Major Histocompatibility Complex (MHC) self-antigens are positively selected into single-positive CD4+ or CD8+ T cells in the thymus. Those CD8+ T cells that survive and mature after activation become cytotoxic cells, expressing T-cell receptors (TCRs) capable of recognizing specific antigenic peptides bound to Class I MHC molecules and the glycoprotein CD8. Affinity between the CD8 protein and the MHC molecule helps to keep the cytotoxic T cell and target closely bound during antigen specific activation. Once activated, CD8+ T cells are generally classified as having a predefined cytotoxic role within the immune system and undergo IL-2 induced clonal expansion, which increases the number of cells specific for the target antigen. The CD8+ T cells then travel throughout the body in search of antigen-positive somatic/tumor cells. 产品信息 检测方法 Luminex xMAP 配置 Design your multiplex kit by choosing analytes. 精度（%） - Intra-assay:< 10
- Inter-assay: < 20
板面类型 MAGNETIC Cytokines/Chemokines 应用 应用 The analytes available for this multiplex kit are: GM-CSF, sCD137, IFNγ, sFas, sFasL, Granzyme A, Granzyme B, IL-2, IL-4, IL-5, IL-6, IL-10, IL-13, MIP-1α, MIP-1β, TNF-α, Perforin. 主要应用 - Multiplexing
应用说明 • This is an overnight incubation assay requiring 25 µL of neat plasma or serum, or 25 μL cell culture supernatant per well.
• This kit may be used for the analysis of all or any combination of the Human CD8+ T-Cell Panel analytes in serum, plasma or tissue/cell lysate and culture supernatant.生物信息 特异性 Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible. 品种反应性 Human 可用的分析物 - Perforin
- GM-CSF
- sCD137
- IFNγ
- sFas
- sFasL
- Granzyme A
- Granzyme B
- IL-2
- IL-4
- IL-5
- IL-6
- IL-10
- IL-13
- MIP-1α
- MIP-1β
- TNF-α
理化信息 灵敏度 - Refer to kit protocol for sensitivities of individual biomarkers.
稀释的线性 % 标准曲线范围 - Perforin: 10 – 50,000 pg/mL
- GM-CSF: 4 – 15,000 pg/mL
- sCD137: 2 – 10,000 pg/mL
- IFNγ:1 – 5,000 pg/mL
- IL-10: 5 – 20,000 pg/mL
- Granzyme A: 20 – 100,000 pg/mL
- IL-13: 2 – 7,500 pg/mL
- Granzyme B: 1 – 5,000 pg/mL
- sFas: 400 – 1,650,000 pg/mL
- IL-2: 2 -7,500 pg/mL
- IL-4: 2 – 10,000 pg/mL
- IL-5: 1 – 5,000 pg/mL
- IL-6: 0.4 – 1,500 pg/mL
- sFasL: 10 – 50,000 pg/mL
- MIP-1α: 1 -3,500 pg/mL
- MIP-1β: 7 – 30,000 pg/mL
- TNFα: 0.5 – 2,000 pg/mL
产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Recommended storage for kit components is 2 - 8°C. 包装信息 数量 96-well plate
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