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描述 产品目录编号 14-349 品牌系列 Upstate
商名 - Upstate
描述 MAPKAP Kinase 2 Protein, unactive, 50 µg 背景信息 Recombinant human MAPKAP Kinase 2 (MAPKAP-K2), residues 46-400, A399G, containing an N-terminal GST tag
MAPKAP Kinase 2 has been implicted as a cell cycle checkpoint kinase, joining the ranks of Chk1 and Chk2 as critical regulators of the DNA damage response in mammalian cells in recent studies (Manke, et al, 2005, and Abraham, 2005). This research indicates that MAPKAP Kinase 2, a serine/threonine kinase activated by p38, phosphorylates CDC25 B/C, generating a binding site for 14-3-3 proteins. The checkpoint kinases Chk1 and Chk2 are targets for drug discovery efforts designed to overcome cell cycle arrest due to DNA damage induced by chemotherapeutic agents. Overriding the checkpoint kinases in cancer cells exposed to DNA damaging agents leads to catastrophic failure of cell division and apoptosis.
The new role of MAPKAP Kinase 2 in complementing the function of Chk1 and Chk2 suggests a new target for drug discovery efforts.应用 应用 Unactive, For use in Kinase Assays. 主要应用 - Kinase Assay
生物信息 来源 Expressed in E.coli 品种 Human 特异活性 For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. Entrez基因编号 - NM_032960.2
- NM_004759.3
Entrez基因汇总 This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene. 基因符号 - MAPKAPK-2
- MAPKAPK2
- MK2
蛋白靶 MAPKAP-K2 纯化方法 Glutathione-agarose 靶子系列 CAMK UniProt编号 - P49137
UniProt汇总 FUNCTION: SwissProt: P49137 # Its physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity.
SIZE: 400 amino acids; 45568 Da
SUBUNIT: Interacts with PHC2.
TISSUE SPECIFICITY: Expressed in all tissues examined.
PTM: Phosphorylated and activated by MAP kinase.
SIMILARITY: SwissProt: P49137 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. & Contains 1 protein kinase domain.产品使用声明 质量保证 Routinely evaluated by phosphorylation of MAPKAP-K2 substrate peptide 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 6 months at -20°C 包装信息 数量 50 µg
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