merck millipore,默克密理博,GF111,Transforming Growth Factor-β1 Protein, Recombinant human

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merck millipore,默克密理博,GF111,Transforming Growth Factor-β1 Protein, Recombinant human
产品名称：Transforming Growth Factor-β1 Protein, Recombinant human
产品型号：GF111
Human Transforming Growth Factor-beta1 (TGF-beta1) is a member of a superfamily of homologous that regulate the proliferation & differentiation of normal & transformed cells.

merck millipore,默克密理博,GF111,Transforming Growth Factor-β1 Protein, Recombinant human

产品介绍

merck millipore,默克密理博,GF111,Transforming Growth Factor-β1 Protein, Recombinant human

重要规格表

主要应用	品种	来源	纯度
CULT	Human	CHO cells	Greater than 98% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per μg (1EU/μg) of TGF-beta1

描述
产品目录编号	GF111
Replaces	GF056
品牌系列	Chemicon®
商名	Chemicon
描述	Transforming Growth Factor-β1 Protein, Recombinant human
概述	Human Transforming Growth Factor-beta1 (TGF-beta1) is a member of a superfamily of homologous that regulate the proliferation and differentiation of normal and transformed cells. Human TGF-beta1 is a 25 kDa disulfide-linked, homodimeric protein with each subunit containing 112 amino acid residues.
Alternate Names	TGF-beta1

产品信息
演示	Sterile Filtered through a 0.2 micron filter. Lyophilized with no additives.

应用
应用	Human Transforming Growth Factor-beta1 (TGF-beta1) is a member of a superfamily of homologous that regulate the proliferation & differentiation of normal & transformed cells.
主要应用	Cell Culture

生物信息
纯度	Greater than 98% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per μg (1EU/μg) of TGF-beta1
来源	CHO cells
品种	Human
特异活性	Biological activity was determined by the ability of the recombinant protein to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells. The ED(50) was found to be <= 0.05 ng/mL, corresponding to a specific activity of >= 2 x 10e7 units/mg.
Entrez基因编号	NM_000660.3
Entrez基因汇总	TGFB is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. TGFB acts synergistically with TGFA (MIM 190170) in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB and almost all of them have specific receptors for this peptide. TGFB1, TGFB2 (MIM 190220), and TGFB3 (MIM 190230) all function through the same receptor signaling systems.[supplied by OMIM]
基因符号	DPD1 TGFB1 CED TGF-beta-1 TGFB
UniProt编号	P01137
UniProt汇总	FUNCTION: SwissProt: P01137 # Multifunctional protein that control proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGFB1 and essentially all of them have specific receptors for this protein. It regulates the actions of many other growth factors and determines a positive or negative direction of their effects. It plays an important role in bone remodeling. It is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. SIZE: 390 amino acids; 44341 Da SUBUNIT: The inactive form consists of a TGFB1 homodimer non- covalently linked to a latency-associated peptide (LAP) homodimer. The inactive complex can contain a latent TGFB1-binding protein. The active form is a homodimer of mature TGFB1; disulfide-linked. Heterodimers of TGFB1/TGFB2 have been found in bone. Interacts with CD109 and DPT. SUBCELLULAR LOCATION: Secreted. TISSUE SPECIFICITY: Highly expressed in bone. PTM: Glycosylated. & The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive. DISEASE: SwissProt: P01137 # Defects in TGFB1 are the cause of Camurati-Engelmann disease (CED) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CED is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision. SIMILARITY: SwissProt: P01137 ## Belongs to the TGF-beta family.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. Storage/Handling: The lyophilized powder, though stable at room temperature, is best stored at -20°C to -80°C until expiration on the vial label. Reconstituted stock solutions of TGF-1 should be stored in undiluted aliquots at -20C to -80°C for up to 10 months. Avoid repeated freezing and thawing.

储存和货运信息

存储条件

Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage/Handling: The lyophilized powder, though stable at room temperature, is best stored at -20°C to -80°C until expiration on the vial label. Reconstituted stock solutions of TGF-1 should be stored in undiluted aliquots at -20C to -80°C for up to 10 months. Avoid repeated freezing and thawing.