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merck millipore,默克密理博,FCCS025108,FlowCellect™ Bcl-2 Activation Dual Detection Kit
产品名称：FlowCellect™ Bcl-2 Activation Dual Detection Kit
产品型号：FCCS025108
This FlowCellect Bcl-2 Activation Dual Detection Kit for Flow Cytometry includes two directly conjugated antibodies: Anti-Bcl-2-Alexa Fluor 488 & Anti-pBcl-2 (Serine 70)-PE.
merck millipore,默克密理博,FCCS025108,FlowCellect™ Bcl-2 Activation Dual Detection Kit
- 产品介绍
描述 产品目录编号 FCCS025108 商名 - FlowCellect
描述 FlowCellect™ Bcl-2 Activation Dual Detection Kit 概述 Millipore’s FlowCellect™ Bcl-2 Activation Dual Detection kit includes two directly conjugated antibodies: Anti-Bcl-2-Alexa Fluor® 488 and Anti-pBcl-2 (Serine 70)-PE, along with optimized fixation, permeabilization, wash, and assay buffers necessary for cell preparation and analysis. Millipore’s FlowCellect™ Activation Dual Detection Kit contains sufficient reagents for 25 2-color samples. Detailed assay instructions are included to assist in your analysis and to ensure that the correct cell concentration is obtained during acquisition of sample data.
Millipore’s FlowCellect™ Bcl-2 Activation Dual Detection Kit is designed to enable a researcher a quick and easy way to detect total Bcl-2 expression as well as the phosphorylation of Bcl-2 at serine 70 simultaneously. Millipore’s FlowCellect™ Activation Dual Dectection Kit was developed and tested using Jurkat cells treated with staurosporine, but the kit can be used with other human cell lines to determine the effect of other chemical reagents on Bcl-2 expression and phosphorylation.Alternate Names - Flow Cellect
背景信息 The signaling pathways that regulate apoptosis are important in understanding tissue homeostasis, the development of the immune system, and therapies for cancer. Bcl-2 is a member of the Bcl-2 family that regulates apoptosis by regulating the proteins that control mitochondrial membrane permeability.
The Bcl-2 family consists of two protein sub-groups, the anti-apoptotic proteins and the pro-apototic proteins (3). Bcl-2 is an anti-apoptotic family member that binds to pro-apoptotic family members, such as Bax to sequester them and inhibit insertion into the mitochondrial membrane. When cells receive an apoptotic signal, Bcl-2 releases Bax, allowing Bax to form a complex on the mitochondrial membrane that releases cytochrome C into the cytoplasm leading to caspase activation and cell death.
Bcl-2 has been shown to be regulated at the level of phosphorylation by JNK at a multi-site phosphorylation loop which includes serine 70. Phosphorylation of Bcl-2 at Serine 70 has been shown to be important in both apoptosis and in autophagy.产品信息 部件 - 6. 1X Permeabilization Buffer: (Part No. CS203284) One bottle containing 13 mL.
- 1. 20X Anti-Bcl-2 Alexa Fluor® 488 Antibody (Part No. CS205126) One vial containing 150 µL antibody.
- 2. 20X Anti-pBcl-2(Ser70) PE Antibody (Part No. CS205125) One vial containing 150 µL antibody.
- 3. Fixation Buffer: (Part No. CS202122) One bottle containing 13 mL.
- 4. 10X Wash Buffer: (Part No. CS202123) One bottle containing 13 mL.
- 5. 5X Assay Buffer: (Part No. CS202124) One 55 mL bottle
检测方法 Fluorescent 应用 应用 This FlowCellect Bcl-2 Activation Dual Detection Kit for Flow Cytometry includes two directly conjugated antibodies: Anti-Bcl-2-Alexa Fluor 488 & Anti-pBcl-2 (Serine 70)-PE. 主要应用 - Flow Cytometry
生物信息 宿主 Mouse 品种反应性 Human 抗体类型 Monoclonal Antibody 产品使用声明 质量保证 Lot to lot in-process testing on all kit components 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
储存和货运信息 存储条件 4-8°C for antibodies and buffers 包装信息 数量 25 Tests / Kit
上一件merck millipore产品：merck millipore,默克密理博,317496,Dual Specificity Protein Phosphatase 1/6 Inhibitor, BCI - CAS 15982-84-0 - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,219421,Cathepsin L Inhibitor I - CAS 108005-94-3 - Calbiochem
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