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merck millipore,默克密理博,ECM555,QCM ECMatrix Cell Invasion Assay, 96-well (8 µm), fluorimetric
产品名称：QCM ECMatrix Cell Invasion Assay, 96-well (8 µm), fluorimetric
产品型号：ECM555
The CHEMICON Cell Invasion Assay Kit provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model.
merck millipore,默克密理博,ECM555,QCM ECMatrix Cell Invasion Assay, 96-well (8 µm), fluorimetric
- 产品介绍
重要规格表
主要应用 检测方法 ACT Fluorescent 描述 产品目录编号 ECM555 品牌系列 Chemicon®
商名 - Chemicon
- QCM
描述 QCM ECMatrix Cell Invasion Assay, 96-well (8 µm), fluorimetric 概述 Also available: Cell Comb™ Scratch Assay! Get biochemical data from a scratch assay!
Introduction
Invasion through the extracellular matrix (ECM) is an important step in tumor metastasis. Cancer cells initiate invasion by adhering to and spreading along the blood vessel wall. Proteolytic enzymes, such as MMP collagenases, dissolve tiny holes in the sheath-like covering (basement membrane) surrounding the blood vessels to allow cancer cells to invade (Egeblad & Werb, 2002).
Microporous membrane inserts are widely used for cell migration and invasion assays. The most widely accepted of which is the Boyden Chamber assay. However, current methods of analysis are time-consuming and tedious, involving cotton swabbing of non-invaded cells on the topside of insert, manual staining and counting. Recently a fluorescence blocking membrane insert was introduced to address these issues; however, this approach requires labeling of the cells with Calcein-AM and extensive washing to remove free Calcein before cell invasion. The effect of this treatment on cell behavior/invasion remains questionable.
The CHEMICON QCM™ 96-well Invasion Assay does not require cell labeling, scraping, washing or counting. The 96-well insert and homogenous fluorescence detection format allows for large-scale screening and quantitative comparison of multiple samples.
In the CHEMICON QCM™ 96-well Invasion Assay, invaded cells on the bottom of the insert membrane are dissociated from the membrane when incubated with Cell Detachment Buffer. These cells are subsequently lysed and detected by the patented CyQuant GR* dye (Molecular Probes) (Gildea et al., 2000). This green-fluorescent dye exhibits strong fluorescence enhancement when bound to cellular nucleic acids (Jones et al., 2001).
*CyQUANT GR® is a registered trademark of Molecular Probes, Inc. The reagent is licensed from Molecular Probes, Inc. and is for use in kits sold by Chemicon International, Inc. for the monitoring of cell invasion and cell migration only.
The CHEMICON Cell Invasion Assay Kit provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model. The kit utilizes ECMatrixTM, a reconstituted basement membrane matrix of proteins derived from the Engelbreth Holm-Swarm (EHS) mouse tumor (Albini et al., 1987; Repesh, 1989; Terranova et al., 1986; Liotta, 1984). We examined the kit's performance using human fibrosarcoma (HT-1080) and non-invasive fibroblasts (NIH3T3).
The CHEMICON QCM™ 96-well Invasion Assay provides a quick and efficient system for quantitative determination of various factors on cell invasion, including screening of pharmacological agents, evaluation of MMPs or other proteases responsible for cell invasion, or analysis of gene function in transfected cells.
In addition, CHEMICON also provides QCM™ 96-well Chemotaxis Cell Migration Assay Systems (8 μm or 3 μm pore), 24-well insert Cell Migration and Invasion Assay Systems, CytoMatrix™ Cell Adhesion strips coated with ECM proteins or anti-integrin antibodies, and QuantiMatrix™ ECM protein ELISA kits.
Test Principle:
The CHEMICON Cell Invasion Assay is performed in a 96 well invasion plate based on the Boyden chamber principle. This plate contains 96 inserts; each insert contains an 8 μm pore size polycarbonate membrane coated with a thin layer of ECMatrixTM. The ECM layer occludes the membrane pores, blocking non-invasive cells from migrating through. Invasive cells, on the other hand, migrate through the ECM layer and cling to the bottom of the polycarbonate membrane. Invaded cells on the bottom of the insert membrane are dissociated from the membrane when incubated with Cell Detachment Buffer and subsequently lysed and detected by CyQuant GR dye.
Application:
The CHEMICON Cell Invasion Assay Kit is ideal for evaluation of invasive tumor cells. Each CHEMICON Cell Invasion Assay Kit contains sufficient reagents for the evaluation of 96 samples. The quantitative nature of this assay is especially useful for large scale screening of pharmacological agents.
The CHEMICON Cell Invasion Assay Kit is intended for research use only; not for diagnostic or therapeutic applications.产品信息 部件 - CyQuant GR Dye®: (Part No. 90132) One vial - 75 μL
- Sterile 96-well Cell Invasion Plate Assembly: (Part No. 90200) One 96-well feeder tray, and one 96-well Cell Invasion Chamber plate with ECMatrixTM -coated inserts.
- 96-well Cell Culture Tray: (Part No. 90129) One 96-well feeder tray.
- Cell Detachment Solution: (Part No. 90131) One bottle - 16 mL.
- 4X Cell Lysis Buffer: (Part No. 90130) One bottle - 16 mL.
检测方法 Fluorescent 应用 应用 The CHEMICON Cell Invasion Assay Kit provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model. 主要应用 - Activity Assay
应用说明 Detection of migrated cells is via
CyQuant GR Dye®.生物信息 品种反应性 Human 产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store kit materials at 2-8°C for up to their expiration date. Do not freeze. 包装信息 数量 1 plate 包装 96 wells
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