merck millipore,默克密理愽
  • merck millipore,默克密理博,05-201,Anti-Fas Antibody (human, activating), clone CH11

  • merck millipore,默克密理博,05-201,Anti-Fas Antibody (human, activating), clone CH11

    产品名称：Anti-Fas Antibody (human, activating), clone CH11
    产品型号：05-201
    Detect Fas using this Anti-Fas Antibody (human, activating), clone CH11 validated for use in Flow Cytometry (FC), Immunocytochemistry (IC) & Western Blot (WB).

    merck millipore,默克密理博,05-201,Anti-Fas Antibody (human, activating), clone CH11

  • 产品介绍
  • merck millipore,默克密理博,05-201,Anti-Fas Antibody (human, activating), clone CH11

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    HWB, FC, ICCMAffinity PurifiedMonoclonal Antibody
    描述
    产品目录编号 05-201
    品牌系列 Upstate
    商名
    • Upstate
    描述 Anti-Fas Antibody (human, activating), clone CH11
    Alternate Names
    • tumor necrosis factor receptor superfamily, member 6
    • APO-1 cell surface antigen
    • CD95 antigen
    • Fas (TNF receptor superfamily, member 6)
    • Fas AMA
    • Fas antigen
    • apoptosis antigen 1
    • apoptosis antigen 1
    • tumor necrosis factor receptor superfamily
    背景信息 Fas/APO-1/CD95 (36 kDa) is a member of the tumor necrosis factor (TNF) receptor superfamily, a family of transmembrane receptors. Fas has been shown to be an important mediator of apoptotic cell death, as well as being involved in inflammation. Binding of the Fas ligand (Fas-L) induces trimerization of Fas in the target cell membrane. Activation of Fas causes the recruitment of Fas-associated protein with death domain (FADD) via interactions between the death domains of Fas and FADD. Procaspase 8 binds to Fas-bound FADD via interactions between the death effector domains (DED) of FADD and pro-caspase 8 leading to the activation of caspase 8. Activated caspase 8 cleaves (activates) other procaspases, in effect beginning a caspase cascade that ultimately leads to apoptosis. Caspases cleave nuclear lamins, causing the nucleus to break down and lose its normal structure. Fas-induced apoptosis can be effectively blocked at several stages by either FLICE-inhibitory protein (FLIP), by Bcl-2, or by the cytokine response modifier A (CrmA).

    Biological Activity
    The antibody demonstrates cytolytic activity on human cells that express Fas. Murine WR19L cells and L929 cells transfected with cDNA encoding human Fas undergo apoptosis in response to this antibody.
    产品信息
    格式 Affinity Purified
    控制
    • Human liver tumor, human breast tumor or Jurkat whole cell lysate, Raji cell lysate.
    演示 Purified mouse monoclonal IgM in buffer containing PBS, pH 7.2, with 50% glycerol. Liquid at -20ºC.
    应用
    应用 Detect Fas using this Anti-Fas Antibody (human, activating), clone CH11 validated for use in Flow Cytometry (FC), Immunocytochemistry (IC) & Western Blot (WB).
    主要应用
    • Western Blotting
    • Flow Cytometry
    • Immunocytochemistry
    应用说明 Western Blot:
    0.5-2 μg/mL of a previous lot detected Fas in a Raji cell lysate.
    Immunocytochemistry:
    5-10 μg/mL of a previous lot detected Fas on HeLa cells fixed with 4% formalin/2% acetic acid.
    Flow cytometry:
    A previous lot of was tested by an independent laboratory using 20 μg/mL of anti-Fas, clone CH11 (Yonehara, S., 1989; Kobayashi, N., 1990).
    生物信息
    免疫原品种 FS-7 (human diploid fibroblast cell line). Clone CH-11.
    克隆 CH11
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 This antibody recognizes the human cell surface antigen Fas, Mr 43 kDa expressed in various human cells, including myeloid cells, T lympho-blastoid cells, and diploid fibroblasts.
    同种型 IgM
    品种反应性 Human
    Species Reactivity Note Proven to react with human.
    This antibody does not recognize TNF, and does not cross-react with mouse Fas. Fas ligand will induce apoptosis in human, mouse and rat systems.
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM_152877
    Entrez基因汇总 The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform.
    基因符号
    • APO-1
    • FAS
    • OTTHUMP00000059646
    • LFG
    • FASTM
    • ALPS1A
    • TNFRSF6
    • CD95
    • FAS1
    • FAIM2
    • APT1
    • NMP35
    纯化方法 Immunoaffinity chromatography
    UniProt编号
    • P25445
    UniProt汇总 FUNCTION: SwissProt: P25445 # Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
    SIZE: 335 amino acids; 37732 Da
    SUBUNIT: Binds DAXX. Interacts with HIPK3. Part of a complex containing HIPK3 and FADD (By similarity). Binds RIPK1 and FAIM2. Interacts with BRE.
    SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted. & Isoform 3: Secreted. & Isoform 4: Secreted. & Isoform 5: Secreted. & Isoform 6: Secreted.
    TISSUE SPECIFICITY: Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
    DOMAIN: SwissProt: P25445 Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.
    DISEASE: SwissProt: P25445 # Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
    SIMILARITY: Contains 1 death domain. & Contains 3 TNFR-Cys repeats.
    产品使用声明
    质量保证 Routinely evaluated by demonstrating cytolytic activity on human cells that express Fas. Murine WR19L cells and L929 cells transfected with cDNA encoding human Fas undergo apoptosis in response to this antibody.

    Apoptosis Assay Analysis:
    15-20 µg/mL of this lot maximally induced apoptosis of human Jurkat cells with 83% mortality after 24 hours of treatment.
    销售限制 This product is not available for sale in Japan.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at -20°C from date of receipt. For maximum recovery of the product, centrifuge the original vial prior to removing the cap.
    包装信息
    数量 50 µg

    merck millipore,默克密理博,05-201,Anti-Fas Antibody (human, activating), clone CH11

上一件merck millipore产品：merck millipore,默克密理博,ST1600,Anti-ADAM12 Mouse mAb (1G3)
下一件merck millipore产品：merck millipore,默克密理博,MAB377B,Anti-NeuN Antibody, clone A60, biotin conjugated


相关产品

merck millipore,默克密理博,ABN301,Anti-HCCS Antibody merck millipore,默克密理博,ABN301,Anti-HCCS Antibody

merck millipore,默克密理博,574715,Syk Inhibitor V - Calbiochem merck millipore,默克密理博,574715,Syk Inhibitor V - Calbiochem

merck millipore,默克密理博,506126,p38 MAP Kinase Inhibitor - CAS 219138-24-6 - Calbiochem merck millipore,默克密理博,506126,p38 MAP Kinase Inhibitor - CAS 219138-24-6 - Calbiochem

merck millipore,默克密理博,SF-824,miR-186-5p; Human, Cyanine 3, RNA Detection Probe,SmartFlare merck millipore,默克密理博,SF-824,miR-186-5p; Human, Cyanine 3, RNA Detection Probe,SmartFlare

merck millipore,默克密理博,ST1600,Anti-ADAM12 Mouse mAb (1G3) merck millipore,默克密理博,ST1600,Anti-ADAM12 Mouse mAb (1G3)

merck millipore,默克密理博,MAB377B,Anti-NeuN Antibody, clone A60, biotin conjugated merck millipore,默克密理博,MAB377B,Anti-NeuN Antibody, clone A60, biotin conjugated

merck millipore,默克密理博,530006,Prion Protein, His•Tag, Human, Recombinant, E. coli merck millipore,默克密理博,530006,Prion Protein, His•Tag, Human, Recombinant, E. coli

merck millipore,默克密理博,400600,L-N⁵-(1-Iminoethyl)ornithine, Dihydrochloride - CAS 159190-44-0 - Calbiochem merck millipore,默克密理博,400600,L-N⁵-(1-Iminoethyl)ornithine, Dihydrochloride - CAS 159190-44-0 - Calbioch...

merck millipore,默克密理博,4700-0073,Guava Antibody Dilution Buffer merck millipore,默克密理博,4700-0073,Guava Antibody Dilution Buffer

merck millipore,默克密理博,ECM555,QCM ECMatrix Cell Invasion Assay, 96-well (8 µm), fluorimetric merck millipore,默克密理博,ECM555,QCM ECMatrix Cell Invasion Assay, 96-well (8 µm), fluorimetric


whatman,沃特曼,nalgene,耐洁,merck millipore,默克密理愽,sartorius,赛多利斯,pall,颇尔
thermo fisher,赛默飞世尔,GE,MN,macherey-nagel,advantec,wheaton,brand,普兰德,welch,airtech


上海羽令过滤器材有限公司版权所有 Copyright ©  www.yl-guolv.com  Powered by 羽令集团—信息技术部