merck millipore,默克密理博,ECM541,ECM Cell Culture Optimization Array (Colorimetric, 96-Wells)

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,ECM541,ECM Cell Culture Optimization Array (Colorimetric, 96-Wells)
产品名称：ECM Cell Culture Optimization Array (Colorimetric, 96-Wells)
产品型号：ECM541
The ECM cell culture Optimization Array enables researchers to identify the best ECM protein for their cell culture environment & also pinpoint the concentration needed to achieve optimal cell growth More>>

merck millipore,默克密理博,ECM541,ECM Cell Culture Optimization Array (Colorimetric, 96-Wells)

产品介绍

merck millipore,默克密理博,ECM541,ECM Cell Culture Optimization Array (Colorimetric, 96-Wells)

重要规格表

检测方法
Colorimetric

描述
产品目录编号	ECM541
描述	ECM Cell Culture Optimization Array (Colorimetric, 96-Wells)
概述	Extracellular matrix (ECM) proteins are produced intracellularly and are subsequently secreted into the surrounding cellular medium, actively regulating a diverse range of cell functions including cell adhesion, differentiation, proliferation, migration, invasion and survival. A primary utility of ECMs in in vitro culture is to promote cellular adhesion while maintaining cell viability and maximizing cell proliferation for downstream cell-based applications. In cases where optimal cell growth conditions are not well defined and critical adhesion protein requirements are unknown, identifying the ideal ECM protein(s) at functionally relevant concentration(s) can be a time-consuming and labor-intensive process. The 96-well, colorimetric ECM Cell Culture Optimization Array provides a multiparametric assay that allows researchers to: (1) quickly identify ECM(s) that best promote cell-type specific adhesion and (2) pinpoint the optimal ECM concentration required to promote maximum adhesion for a particular cell type. How does the ECM Cell Culture Optimization Array work? The foundation of our kit is a 96-well microtiter plate containing several commonly-used ECM proteins. Collagen I, laminin, fibronectin, and vitronectin are arrayed in triplicate with concentrations ranging from 0.125 μg/mL to 20 μg/mL. You simply culture your cells on the plate, and use the included reagents to block, stain, and analyze the adhesion capacity with a plate reader. We have extensively tested and optimized this kit on a variety of cell types including HEK293 cells, and human and rodent neural stem cells.

产品信息
部件	• Assay Buffer: (Part No. 2003646) One bottle - 100 mL • ECM Gradient Array Plate: One 96-well plate with 12 strips • 30%BSA: (Part No. CS201638) One vial - 0.5mL • Cell Stain Solution: (Part No. 90144) One bottle - 20 mL. • Extraction Buffer: (Part No. 90145) One bottle - 20 mL
检测方法	Colorimetric

应用
应用	The ECM cell culture Optimization Array enables researchers to identify the best ECM protein for their cell culture environment & also pinpoint the concentration needed to achieve optimal cell growth conditions.
应用说明	The ECM Cell Culture Optimization Array is the first commercially-available tool of its kind to enable researchers to not only quickly identify the best ECM protein for their cell culture environment, but also pinpoint the concentration needed to achieve optimal cell growth conditions.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	The ECM Gradient Array Plate should be stored in the foil pouch at -70ºC, and used within three months of the date of receipt. The remaining kit components should be stored at 2º to 8ºC.