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merck millipore,默克密理博,ECM205,Millicoat™ ECM Screening Kit, 1 ea. ECM101-ECM105
产品名称：Millicoat™ ECM Screening Kit, 1 ea. ECM101-ECM105
产品型号：ECM205
The MillicoatTM screening kit contains individual 96-well plates for fibronectin, vitronectin, laminin, collagen I & collagen IV.
merck millipore,默克密理博,ECM205,Millicoat™ ECM Screening Kit, 1 ea. ECM101-ECM105
- 产品介绍
重要规格表
主要应用 检测方法 ACT Chromogenic 描述 产品目录编号 ECM205 品牌系列 Chemicon®
商名 - Chemicon
- Millicoat
描述 Millicoat™ ECM Screening Kit, 1 ea. ECM101-ECM105 概述 Cell adhesion plays a major role in cellular communication and regulation, and is of fundamental importance in the development and maintenance of tissues. Scientists are continually examining the adhesion and migration of many diverse cell types on various extracellular matrix (ECM) component proteins. Millicoat™ Cell Adhesion Strips are provided as 12 8-well removable strips in a plate frame for convenience and flexibility in designing assays. The wells in rows A - G have been coated with a human ECM protein. Row H of each strip is coated with BSA which serves as a negative assay control. The MillicoatTM screening kit contains individual 96-well plates for fibronectin, vitronectin, laminin, collagen I and collagen IV. Cells are seeded onto the coated substrate. Subsequently, adherent cells are fixed and stained. Relative attachment is determined using absorbance readings. Alternate Names - Formerly under the CytoMatrix brand name.
产品信息 检测方法 Chromogenic 应用 应用 The MillicoatTM screening kit contains individual 96-well plates for fibronectin, vitronectin, laminin, collagen I & collagen IV. 主要应用 - Activity Assay
应用说明 PROCEDURE:
NOTE: Optimal assay performance is obtained using subconfluent cell cultures. This can be achieved by splitting the cells 1 to 2 days prior to performing the assay.
1. Rehydrate the strips with 200 mL of PBS per well for at least 15 minutes at room temperature. Remove the PBS from the rehydated strips.
2. Prepare a single cell suspension, preferably using a non-enzymatic dissociation buffer. Optimum cell density may be determined by titration of the cells. A common starting range is between 1x10E05 to 1x10E07 cells/mL.
3. Add 100 mL of the diluted cell suspension to each well. Incubate the plate at 37°C for 1 hour in a CO2 incubator. Gently wash the plate 3 times with PBS containing Ca2+/Mg2+ (200 mL/well).
4. Add 100 mL/well of 0.2% crystal violet in 10% ethanol to each well. Incubate for 5 minutes at room temperature. Remove the stain from the wells. Gently wash the plate 3 times with PBS (300 mL/well).
5. Add 100 mL of Solubilization Buffer (A 50/50 mixture of 0.1M NaH2PO, pH 4.5 and 50% ethanol) to each well. Allow strips to incubate and gently shake at room temperature until the cell-bound stain is completely solubilized; approximately 5 minutes.
6. Determine the absorbances at 540 - 570 nm on a microplate reader.生物信息 品种反应性 Human 产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 May be stored at 2-8°C in the foil pouch for at least 3 months. Unused strips may be placed back in the pouch for storage. Ensure that the desiccant remains in the pouch, and that the pouch is securely closed. 包装信息 数量 1 set
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