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品种反应性 主要应用 宿主 格式 抗体类型 R WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB5226-50UL 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-P2X4 Receptor Antibody 产品信息 格式 Affinity Purified 控制 - CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
演示 Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min). 应用 应用 Anti-P2X4 Receptor Antibody detects level of P2X4 Receptor & has been published & validated for use in WB. 主要应用 - Western Blotting
应用说明 Western blot: 1-1.5 μg/mL (1:200:1:300) using ECL
Immunohistochemistry on rat brain sections.
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 A purified peptide of rat P2X4 protein (amino acids 370-388) (Accession P51577). 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes a full-length P2X4 purinergic receptor protein and not with other known proteins tested so far.
SPECIES REACTIVITIES: The immunogen sequence is identical in mouse. Other species have not been tested.品种反应性 Rat 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_002560.2
Entrez基因汇总 The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel with high calcium permeability. The main pharmacological distinction between the members of the purinoceptor family is the relative sensitivity to the antagonists suramin and PPADS. The product of this gene has the lowest sensitivity for these antagonists. Multiple alternatively spliced transcript variants have been identified for this gene although their full-length natures have not been determined. 基因符号 - P2X4
- P2RX4
- P2X4R
UniProt编号 - Q99571
UniProt汇总 FUNCTION: SwissProt: Q99571 # Receptor for ATP that acts as a ligand gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin.
SIZE: 388 amino acids; 43369 Da
SUBUNIT: Homo- or heteropolymers (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: Q99571 ## Belongs to the P2X receptor family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 50 µL
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