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纯度 ≥95% by SDS-PAGE 描述 概述 Recombinant, mouse R-Spondin 4 consisting of amino acids 19-228 and expressed in Chinese Hamster Ovary cells. Able to induce activation of β-catenin response in a Luciferase assay using HEK293T human embryonic kidney cells. R-spondin 4 is useful as a positive modulator of Wnt/β signaling. 产品目录编号 552095 品牌系列 Calbiochem®
同义词 Cristin 4 参考 参考 Hendrickx, M. and L. Leyns. 2008. Develop. Growth Differ. 50, 229.
Ishii, Y., et al. 2008. J. Invest. Dermatol. 128, 867.
Kim, K.A., et al. 2008. Mol. Biol. Cell 19, 2588.
Binnerts, M.E., et al. 2007. Proc. Natl. Acad. Sci. USA 104 14700.
Nam, J.S., et al. 2007. Gene Expr. Patterns 7, 306.
Pemberton, T.J., et al. 2007. Dev. Dyn. 236, 2245.
Bergmann, C., et al. 2006. Am. J. Hum. Genet. 79, 1105.
Blaydon, D.C., et al. 2006. Nat. Genet. 38, 1245.
Nam, J.S., et al. 2006. J. Biol. Chem. 281, 13247.产品信息 形式 Solid 配方 Lyophilized from 0.2 µm sterile-filtered PBS, NaCl, EDTA, BSA. 生物信息 生物活性 ED₅₀ = 5-20 ng/ml in the presence of 10 ng/ml rmWnt-3a, as measured by induction of activation of β-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. 纯度 ≥95% by SDS-PAGE 理化信息 污染物 Endotoxin: <1.0 EU/µg protein 储存和货运信息 货运代码 Ambient Temperature Only
毒性 Standard Handling
储存 -20°C
无需冷冻 No
特殊指南 Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
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