merck millipore,默克密理博,ECM644,In Vitro Vascular Permeability Assay (24-well)

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merck millipore,默克密理博,ECM644,In Vitro Vascular Permeability Assay (24-well)
产品名称：In Vitro Vascular Permeability Assay (24-well)
产品型号：ECM644
This In Vitro Vascular Permeability Assay kit provides an efficient system for evaluating the effects of chemicals & drug compounds on endothelial cell adsorption, transport & permeability.

merck millipore,默克密理博,ECM644,In Vitro Vascular Permeability Assay (24-well)

产品介绍

merck millipore,默克密理博,ECM644,In Vitro Vascular Permeability Assay (24-well)

Replacement Information
Replacement Information	ECM640 NOW AVAILABLE! 96-well (ECM642) Collagen Pre-coated Version

重要规格表

检测方法
Fluorescent

描述
产品目录编号	ECM644
Replaces	ECM640
品牌系列	Chemicon®
商名	Chemicon
描述	In Vitro Vascular Permeability Assay (24-well)
概述	NOW AVAILABLE! In Vitro Vascular Permeability Imaging Assay (17-10398) *Read our application note in Nature Methods!* Introduction: A fundamental requirement for the physiological performance of organs is the formation of diffusion barriers that separate and maintain compartments of different structure. The endothelial cell lining of the internal vasculature defines a semi-permeable barrier between the blood and the interstitial spaces of the body. This barrier is composed of intercellular adherens, tight, and gap junction complexes, as well as desmosomes. Junction substructure components such as connexins, integrins, cadherins, catenins, occludins, desmoplakins, selectins, and platelet endothelial cell adhesion molecule-1 (PECAM-1) all act as interface regulators for paracellular permeability of ions, nutrients, therapeutic agents, and macromolecules. Endothelial cell adhesive characteristics provide strength and stability for neighboring cells and the cellular cytoskeleton by interacting with actin and myosin contractile filaments. Junctional molecules also influence cell signaling and trigger responses that are translated into cell morphology changes and physiological angiogenesis. A multitude of vasoactive cytokines, growth factors, and signal modulators react with endothelial cell substructural components to control permeability. Vascular endothelial growth factor (VEGF), interleukin-1 alpha and beta (IL-1α and IL-1β), tumor necrosis factor-alpha (TNF-α), and interferon-gamma (IFN-γ) have been shown to increase endothelial monolayer permeability. Thrombin stimulation of cytoskeletal signaling pathways has been shown to manipulate cell permeability. Lipopolysaccharide (LPS) induces junction barrier loss and cell detachment by activating protein tyrosine kinases (PTKs) and caspase cleavage reactions. In contrast, junctional adhesion molecule (JAM) decreases permeability by initiating cell adhesion and angiopoietin-1 (Ang-1) can protect endothelial barrier function through regulation of junctional complexes. Disruptions of the barrier integrity are manifested as microvascular hyperpermeability, which is associated with many systemic disease states. Pathological angiogenic disease states include heart disease, diabetes, cancer, stroke, hypertension, arthritis, and Alzheimer’s. Increases in tissue permeability may be caused by weak, hemorrhaging vessels that become oedematous, and intensifies with irregular fluid flow through the vessels. Expanding the knowledge of endothelial junction behavior and the agents that influence that behavior will lead to new therapies for controlling endothelial permeability. An essential ingredient of any in vitro permeability study is an intact, confluent cell monolayer. Endothelial cell monolayers cultured on semi-permeable membranes have been shown to form adherent and tight junctions. The Millipore In Vitro Vascular Permeability Assay kit provides an efficient system for evaluating the effects of chemicals and drug compounds on endothelial cell adsorption, transport, and permeability. Test Principle: The Millipore In Vitro Vascular Permeability Assay is performed in a 24-well receiver plate with 24 individual hanging cell culture inserts. The inserts contain 1 µm pores within a transparent polyethylene terephthalate (PET) membrane. Each insert has been pre-coated with an optimized concentration of type I rat-tail collagen. The high pore density membranes permit apical and basolateral access of cells to media and permeability molecules of interest. Within Millipore’s In Vitro Vascular Permeability Assay, endothelial cells are seeded onto the collagen-coated inserts. An endothelial monolayer forms in several days, which occludes the membrane pores. The cell monolayer is then treated with cytokines, growth factors, or other compounds of interest. After treatment, a high molecular weight FITC-Dextran is added on top of the cells, allowing the fluorescent molecules to pass through the endothelial cell monolayer at a rate proportional to the monolayer’s permeability. The extent of permeability can be determined by measuring the fluorescence of the receiver plate well solution.

产品信息
部件	Black Plate, 96-Well Opaque: One 96-well plate. 24-Well Permeability Insert Assembly, Collagen-coated: One 24-well receiver tray containing 24 porous cell culture inserts pre-coated with type I rat-tail collagen. Receiver Tray, 24-Well: Two 24-well plates. FITC-Dextran Solution: One vial containing 250 µL. Forceps: One set of forceps. Cell Stain: One bottle containing 10 mL.
检测方法	Fluorescent

应用
应用	This In Vitro Vascular Permeability Assay kit provides an efficient system for evaluating the effects of chemicals & drug compounds on endothelial cell adsorption, transport & permeability.
应用说明	The Millipore® In Vitro Vascular Permeability Assay kit provides an efficient system for evaluating the effects of chemicals and drug compounds on endothelial cell adsorption, transport, and permeability. It is ideal for measuring compounds that may disrupt or protect an endothelial monolayer. Each In Vitro Vascular Permeability Assay Kit contains sufficient reagents for the evaluation of 24 samples. The In Vitro Vascular Permeability Assay Kit is intended for research use only, not for diagnostic or therapeutic applications.

应用

This In Vitro Vascular Permeability Assay kit provides an efficient system for evaluating the effects of chemicals & drug compounds on endothelial cell adsorption, transport & permeability.

应用说明

The Millipore® In Vitro Vascular Permeability Assay kit provides an efficient system for evaluating the effects of chemicals and drug compounds on endothelial cell adsorption, transport, and permeability. It is ideal for measuring compounds that may disrupt or protect an endothelial monolayer. Each In Vitro Vascular Permeability Assay Kit contains sufficient reagents for the evaluation of 24 samples. The In Vitro Vascular Permeability Assay Kit is intended for research use only, not for diagnostic or therapeutic applications.

生物信息
品种反应性	All

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.