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merck millipore,默克密理博,CKT2MAG-97K,MILLIPLEX MAP Canine Kidney Toxicity Panel 2 - Toxicity Multiplex Assay
产品名称：MILLIPLEX MAP Canine Kidney Toxicity Panel 2 - Toxicity Multiplex Assay
产品型号：CKT2MAG-97K
The analytes available for this multiplex kit are: Albumin, β-2-microglobulin (B2M), Trefoil Factor 3 (TFF-3), Retinol Binding Protein 4 (RBP4)
merck millipore,默克密理博,CKT2MAG-97K,MILLIPLEX MAP Canine Kidney Toxicity Panel 2 - Toxicity Multiplex Assay
- 产品介绍
- merck millipore,默克密理博,CKT2MAG-97K,MILLIPLEX MAP Canine Kidney Toxicity Panel 2 - Toxicity Multiplex Assay
重要规格表
可用的分析物 品种反应性 主要应用 检测方法 Retinol Binding Protein 4 (RBP4), Albumin, β-2-Microglobulin, Trefoil Factor 3 (TFF-3) Ca Mplex Luminex xMAP 描述 产品目录编号 CKT2MAG-97K 商名 - MILLIPLEX
描述 MILLIPLEX® MAP Canine Kidney Toxicity Panel 2 - Toxicity Multiplex Assay 概述 The MILLIPLEX® MAP Canine Kidney Toxicity Panel 2 contains all the components necessary to measure the following four biomarkers in any combination using Luminex® xMAP® technology: Albumin, β-2-microglobulin (B2M), Trefoil Factor 3 (TFF-3), Retinol Binding Protein 4 (RBP4). The kit uses a 96-well format, contains a lyophilized standard cocktail, two quality controls and can measure up to 38 urine samples in duplicate. 背景信息 As one of the leading causes of drug candidate attrition, toxicity research is receiving more attention, especially in the search of sensitive, organ-specific toxicity biomarkers.
In pharmaceutical and chemical industries, renal function is routinely assessed during toxicity evaluations. The importance of the kidney as a central detoxification organ leads to a high exposure of renal tissue to drugs, reactive metabolites, and environmental compounds. Traditional markers for assessing renal toxicity, such as blood urea nitrogen (BUN) and serum creatinine, are insensitive and non-specific. For improved, non-invasive detection of acute nephrotoxicity, a panel of novel urinary kidney biomarkers has been identified and validated for preclinical studies in relevant animal species.
EMD Millipore provides a valuable research assay to investigate multiple biomarkers of kidney injury in canine urine samples using the Luminex xMAP® instrument platform. The MILLIPLEX® MAP Canine Kidney Toxicity Panel 2 offers simultaneous analysis of any combination of the following analytes: Albumin*, β-2-microglobulin (B2M)*, Trefoil Factor 3 (TFF-3)*, Retinol Binding Protein 4 (RBP4)*.
*Urinary albumin, B2M, TFF-3 and RBP4 are biomarkers listed in the Predictive Safety Testing Consortium (PSTC) project pipeline which have a strong translational role in drug safety testing.产品信息 部件 - 2 Foil Plate Sealers
- Canine Kidney Toxicity Panel 2 Standard, 1 vial, lyophilized
- Canine Kidney Toxicity Panel 2 Control 1, 1 vial, lyophilized
- Canine Kidney Toxicity Panel 2 Control 2, 1 vial, lyophilized
- Assay Buffer, 2 bottles, 30 mL
- Wash Buffer, 10X (0.05% Proclin), 2 bottlesl, 30 mL
- Canine Kidney Toxicity Panel 2 Detection Antibodies, 1 bottle, 5.5 mL
- Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL
- Mixing Bottle, 1 bottle
- Sample Plate, 96 well format, 1 plate
检测方法 Luminex xMAP 配置 Design your multiplex kit by choosing analytes. 精度（%） - Intra-assay: <10
- Inter-assay: <15
板面类型 MAGNETIC Toxicity 应用 应用 The analytes available for this multiplex kit are: Albumin, β-2-microglobulin (B2M), Trefoil Factor 3 (TFF-3), Retinol Binding Protein 4 (RBP4) 主要应用 - Multiplexing
应用说明 • Customers should determine the optimal dilution factor for their samples. Our recommendations are based on urine samples from normal subjects.
• This assay requires 25 µL of 1:500 diluted urine sample per well in kit Assay Buffer (please dilute per protocol instructions).
• This is a 2 hour assay.生物信息 特异性 Approximately 5% cross-reactivity may be observed between B2M beads and TFF-3 protein. No other significant cross-reactivity was observed for other analytes in this panel. 品种反应性 Canine 可用的分析物 - Retinol Binding Protein 4 (RBP4)
- Albumin
- β-2-Microglobulin
- Trefoil Factor 3 (TFF-3)
化验面板 Everything you need in a single kit. 交叉反应性 Approximately 5% cross-reactivity may be observed between B2M beads and TFF-3 protein. No other significant cross-reactivity was observed for other analytes in this panel. 准确度 - 96% - 102%
理化信息 灵敏度 - See kit protocol for individual analyte sensitivities.
- Albumin: 12.48 ng/mL (MinDC +2SD)
- All other analytes: 0.016 - 0.026 ng/mL (MinDC +2SD)
稀释的线性 Contact technical support for this information. 标准曲线范围 - RBP4: 0.01 – 50 ng/mL
- Albumin: 12.21 – 50,000 ng/mL
- B2M: 0.02 – 100 ng/mL
- TFF-3: 0.02 – 100 ng/mL
产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Recommended storage for kit components is 2 - 8°C. 包装信息 数量 96-well plate
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