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主要应用 Entrez基因编号 品种 Uni Prot编号 ACT NM_032983.2, NM_032982.2 Human P42575 描述 产品目录编号 CC127 品牌系列 Chemicon®
商名 - Chemicon
描述 Caspase 2, recombinant human active 概述 Caspase-2 (also known as ICH-1, NEDD-2) is a member of the interleukin-1beta converting enzyme (ICE) family of cystein proteases. Similar as other caspases, caspase-2 also exists in cells as an inactive proenzyme. During apoptosis, procaspase-2 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases. The processed form of caspase-2 consists of large (19 kDa) and small (12 kDa) subunits which associate to form the active enzyme. The active recombinant human caspase-2 was expressed in E. coli. The expressed caspase-2 spontaneously undergoes autoprocessing to yield the subunits characteristic of the native enzyme. The recombinant caspase-2 preferentially cleaves the substrates consisting of consensus sequence VDVAD (e.g., VDVAD-AFC and VDVAD-pNA). The recombinant enzyme can also be used as a positive control in caspase assays or in determining the specificity of caspase substrates.Recommended usage: 0.5-1 unit for fluorometric caspase assays and 2-4 units for colorimetric caspase assays. Optimal working dilutions must be determined by end user. 产品信息 演示 Partially purified recombinant caspase 2 with a full length HIS-6-tag. Approximately 10-20% caspase 2 protein. The remainder is E.Coli proteins. Approximately 0.8-2 mg total protein/U of activity; E.Coli proteins have no reactivity. Lyophilized. Reconstitute in 25 μL PBS containing 15% glycerol. 应用 主要应用 - Activity Assay
生物信息 纯度 Partially purified recombinant caspase 3 with a full length HIS-6-tag. Approximately 10-20% caspase 2 protein. The remainder is E.Coli proteins. Approximately 0.8-2 mg total protein/U of activity; E.Coli proteins have no reactivity. 品种 Human 特异活性 25U: Where one unit of the recombinant caspase-2 is the enzyme activity that cleaves 1 nmol of the caspase substrate VDVAD-pNA (pNA: pnitroanaline) per hour at 37oC in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT. Entrez基因编号 - NM_032983.2
- NM_032982.2
Entrez基因汇总 This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The proteolytic cleavage of this protein is induced by a variety of apoptotic stimuli. Alternative splicing of this gene results in multiple transcript variants that encode different isoforms. 基因符号 - Caspase-2 subunit p12].
- CASP2
- ICH1
- NEDD2
- NEDD-2
- ICH-1L/1S
- CASP-2
- ICH-1L
- EC 3.4.22.55 [Contains: Caspase-2 subunit p18
- Caspase-2 subunit p13
UniProt编号 - P42575
UniProt汇总 FUNCTION: SwissProt: P42575 # Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.
SIZE: 452 amino acids; 50685 Da
SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a p18 subunit and a p12 subunit. Interacts with LRDD.
TISSUE SPECIFICITY: Expressed in larger amounts in the embryonic lung, liver and kidney than in the heart and brain. In the adults higher level expression is seen in the placenta, lung, kidney, pancreas than in the heart, brain, liver and skeletal muscle.
PTM: The mature protease can process its own propeptide, but not that of other caspases.
SIMILARITY: SwissProt: P42575 ## Belongs to the peptidase C14 family. & Contains 1 CARD domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain unopened and rehydrated product at -70°C in undiluted, freeze-thaw protected, tightly sealed aliquots. Avoid repeated freeze/thaw cycles. Product stable for 6 months from date of purchased if handled appropriately. 包装信息 数量 25 units
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