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检测方法 Fluorescence 描述 概述 A convenient kit for the identification of changes in the plasma membrane that occur during apoptosis. In apoptotic cells, phosphoatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane, allowing for the detection of PS on the cell surface. In the presence of Ca 2+, Annexin V exhibits a high affinity for PS and binds cells with exposed PS. Annexin V, when conjugated to a fluorophore, can be used to monitor apoptopic cells using flow cytometry or immunofluorescence microscopy. 产品目录编号 CBA059 品牌系列 Calbiochem®
参考 参考 Darzynkiewicz, Z., et al. 1997. Cytometry 27, 1.
Frey, T. 1997. Cytometry 28, 253;
Boersma, A.W.M., et al. 1996. Cytometry 24, 123.
White, E. 1996. Genes and Devel. 10, 1.
Martin, S.J., et al. 1995. J. Exp. Med. 182, 1545.
Koopman, G., et al. 1994. Blood 84, 1415.
Wyllie, A.H.,1993. Br. J. Cancer 67, 205.
Darzynkiewicz, Z., et al. 1992. Cytometry 13, 795.
Fadok, V.A., et al. 1992. J. Immunology 148, 2207.
Schmid, I., et al. 1992. Cytometry 13, 204.
Kerr, J.F.R., et al. 1972. Cancer 26, 239.产品信息 检测方法 Fluorescence 形式 20 Tests 格式 Flow cytometry or fluorescence microscopy 试剂盒包含 Annexin V-FITC, Binding Buffer, Propidium Iodide and a user protocol. 应用 主要应用 Flow Cytometry
Immunofluorescence
生物信息 化验时间 30 min 样品类型 Intact cells 产品使用声明 预定用途 The Calbiochem® Annexin V-FITC Apoptosis Detection Kit II is a non-isotopic system that can be used to detect phosphatidylserine on the outer leaflet of the cell membrane of apoptotic cells using flow cytometry or fluorescence microscopy. 储存和货运信息 货运代码 Blue Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
储存 +2°C to +8°C
存储条件 Upon arrival store the entire contents of the kit at 4°C. 无需冷冻 No
补充信息 试剂盒包含 Annexin V-FITC, Binding Buffer, Propidium Iodide and a user protocol.
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