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merck millipore,默克密理博,HTSHEKT-1LT,ChemiBrite™ HEKT Parental Control 1 Frozen Cells
产品名称：ChemiBrite™ HEKT Parental Control 1 Frozen Cells
产品型号：HTSHEKT-1LT
This product has been designed to use as a control for experiments performed with certain GPCR expressing ChemiBrite frozen cells, typically those that natively couple to Gq/11 proteins.
merck millipore,默克密理博,HTSHEKT-1LT,ChemiBrite™ HEKT Parental Control 1 Frozen Cells
- 产品介绍
重要规格表
应用说明 品种 宿主细胞 Calcium Flux Assays Human HEKT 描述 产品目录编号 HTSHEKT-1LT 商名 - ChemiBrite
描述 ChemiBrite™ HEKT Parental Control 1 Frozen Cells 背景信息 Millipore’s ChemiBrite cells co-express a GPCR along with a novel variant of clytin, a calcium-activated photoprotein, to enable sensitive luminescent detection of ligand-induced calcium flux. The ChemiBrite version of clytin contains a mutation that increases its affinity for calcium to a level that permits detection of cytosolic calcium in many cells with greater sensitivity than other mitochondrially expressed photoproteins. Luminescent calcium assays offer several advantages over fluorescent calcium assays including; lower substrate cost, increased sensitivity, and lack of interference from fluorescent compounds.
Like Millipore’s Ready-to-Assay frozen cells, ChemiBrite frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
This product has been designed to use as a control for experiments performed with certain GPCR expressing ChemiBrite frozen cells, typically those that natively couple to Gq/11 proteins. Millipore’s control ChemiBrite cells are made by transient transfection of HEK293T cells with optimized quantities of plasmid encoding clytin and then cryopreserving the cells at an optimal time post-transfection. These conditions closely match those that are applied to ChemiBrite frozen cells transfected with GPCRs. Upon thaw, recovery, and loading, the cells are ready for luminescent analysis to determine if the host cells respond to chemical or biological substances.产品信息 部件 - Vial contains 1 ml of mycoplasma-free cells
应用 应用 This product has been designed to use as a control for experiments performed with certain GPCR expressing ChemiBrite frozen cells, typically those that natively couple to Gq/11 proteins. 应用说明 Calcium Flux Assays 生物信息 品种 Human 宿主细胞 HEKT 靶子系列 Control 产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
包装信息 数量 1 vial
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