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检测方法 Fluorometric 描述 概述 The InnoCyte™ Laminin-Based 96-well Cell Invasion Assay is designed to determine the invasive capacity of cells using laminin as a significant barrier to invasion or to screen potential anti-metastatic agents in vitro in a convenient, high-throughput, 96-well format. The 8 μm pore size is appropriate for all types of adherent cells such as epithelial, endothelial, or fibroblast cells. The assay is based on the Boyden-chamber principle. Each well of the 96-well cell culture insert contains a polycarbonate membrane with an 8 μm pore size that is coated with a laminin protein layer. The laminin layer forms an effective barrier that prevents non-invasive cells from passing through the 8 μm pores. Invasive cells that can degrade the laminin layer will migrate through the membrane and attach to the underside of the membrane. The invasive cells are dislodged from the underside of the cell culture insert and stained with a fluorescent dye in a single step. Fluorescence is determined using a fluorimeter with an excitation wavelength of ~485 nm and an emission wavelength of ~520 nm. 产品目录编号 CBA043 品牌系列 Calbiochem®
参考 参考 Caroll, D.K., et al. 2006. Nat. Cell Biol. 8, 551.
Ekblom, P., et al. 2003. Matrix Biol. 22, 35.
Hood, J.D. and Cheresh, D.A. 2002. Nat. Rev. Cancer 2, 91.
Aumailley, M., et al. 1998. J. Anat. 193, 1.
Aumailley, M., et al. 1996. J. Invest. Dermatol. 106, 209.
Burgeson, et al. 1994, Matrix Biol. 14, 209.
Aznavoorian, S., et al. 1993. Cancer 71, 1368.
Stetler-Stevenson, W. G., et al. 1993. Annu. Rev. Cell Biol. 9, 541.产品信息 检测方法 Fluorometric 形式 96 Tests 格式 96-well plate 试剂盒包含 96-Well Laminin-Coated 96-Well Cell Invasion Chamber, Black 96-Well Plate, Additional Tray , Calcein-AM Solution, Cell Detachment Buffer, and a user protocol. 应用 应用参考 Caroll, D.K., et al. 2006. Nat. Cell Biol. 8, 551. 生物信息 化验时间 14-26 h 样品类型 Cells 产品使用声明 预定用途 The InnoCyte™ Laminin-Based 96-well Cell Invasion Assay is designed to determine the invasive capacity of cells using laminin as a significant barrier to invasion or to screen potential anti-metastatic agents in vitro in a convenient, high-throughput, 96-well format. The 8 µm pore size is appropriate for all types of adherent cells such as epithelial, endothelial, or fibroblast cells. 储存和货运信息 货运代码 Blue Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
储存 Multiple storage requirements
存储条件 Upon arrival store the Calcein-AM at -20°C and the remaining components at 4°C. 避光 Protect from light
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
补充信息 试剂盒包含 96-Well Laminin-Coated 96-Well Cell Invasion Chamber, Black 96-Well Plate, Additional Tray , Calcein-AM Solution, Cell Detachment Buffer, and a user protocol.
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