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检测方法 Fluorometric 描述 概述 A convenient assay for the determination of the relative attachment of adherent cell lines to collagen type IV. Cells are seeded onto the collagen type IV coated plates followed by determination of relative cell attachment using a fluorescent dye. 产品目录编号 CBA013 品牌系列 Calbiochem®
参考 参考 Caroll, D.K., et al. 2006. Nat. Cell Biol. 8, 551.
Hynes, R.O., et al. 1999. Proc. Natl. Acad. Sci. USA96, 2588.
Ohashi, T., et al. 1999. Proc. Natl. Acad. Sci USA 96, 2153.
Grande, J.P., et al. 1997. J. Lab. Clin. Med. 130, 476.
Nelson, P.R., et al. 1997. Vasc. Surg. 26, 104.
Horowitz, A.F., et al. 1996. Trends Cell Biol. 6, 460.
Hynes, R.O., et al. 1990. Fibronectins, Springer-Verlag, New York.
Preissner, K.T., et al. 1990. J. Biol. Chem. 265, 18490.产品信息 检测方法 Fluorometric 形式 72 Tests 格式 96-well plate 试剂盒包含 Coated 96-Well Plate, Fluorescent Dye, D-PBS, and a user protocol. 应用 应用参考 Caroll, D.K., et al. 2006. Nat. Cell Biol. 8, 551. 生物信息 化验时间 2.5 h 样品类型 Adherent cultured cells 理化信息 最大排放 ~520 nm 最大激励 ~485 nm 产品使用声明 预定用途 The Innocyte™ Cell Adhesion Microplate Assays are designed for the determination of the relative attachment of adherent cell lines to extracellular matrix proteins such as Human Fibronectin (Cat. No. CBA011), Human Vitronectin (Cat. No. CBA012), and Human Collagen IV (Cat. No. CBA013). Cells are seeded onto a coated substrate, which is followed by the determination of relative cell attachment using a fluorescent dye. 储存和货运信息 货运代码 Blue Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
储存 Multiple storage requirements
存储条件 Upon arrival store the Calcein-AM at -20°C and the remaining components at 4°C. 避光 Protect from light
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
补充信息 试剂盒包含 Coated 96-Well Plate, Fluorescent Dye, D-PBS, and a user protocol.
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