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宿主 Rb 描述 概述 Recognizes the ~400 kDa BRCA2 protein in MCF-7 and HeLa cells and breast carcinoma. 产品目录编号 CA1033 品牌系列 Calbiochem®
同义词 Anti-Breast Cancer Susceptibility Protein 应用数据 
Detection of human BRCA2 by immunoprecipitation and immunoblotting. Samples: Whole cell lysate from MCF-7 cells (750 µg) (lower panel) and HeLa cells (750 µg) (upper panel). Antibody for IP: Rabbit IgG Control (5µg, lane 1) and Anti-BRCA2 Rabbit pAb (Cat. No. CA1033) (5 µg, lane 2; 10 µg, lane 3; and 15 µg, lane 4). Conditions for immunoblotting: Samples: Whole cell lysate (100 µg) from MCF-7 cells and HeLa cells (lane 5, lower and upper panels, respectively) and BRCA2 IP samples (lanes 1-4). Primary antibody: Anti-BRCA2 (Ab-1) Mouse mAb (2B) (Cat. No. OP95) (2 µg/ml). Detection: chemiluminescence.
Detection of human BRCA2 by staining paraffin sections. Sample: Breast carcinoma tissue (formalin-fixed, paraffin-embedded, heat-pretreated in 10 mM sodium citrate buffer). Primary antibody: Rabbit IgG Control (10 µg/ml, left panel) and Anti-BRCA2 Rabbit pAb (Cat. No. CA1033) (10 µg/ml, right panel). Detection: DAB with methyl green counterstain.参考 参考 First International Workshop on the Function of BRCA1 and BRCA2. 11-12 September, 1997. Churchill College, Cambridge, UK.
Ludwig, T., et al. 1997. Genes Devel. 11, 122.
Mizuta, R., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 6927.
Rajan, J.V., et al. 1997. Dev. Biol. 184, 385.
Sharan, S.K., et al. 1997. Nature 386, 804.
Suzuki, A., et al. 1997. Genes Devel. 11, 1242.
Canon-Albright, L.A. and Skolnick, M.H. 1996. Semin. Oncol. 23, 1.
Goggins, M., et al. 1996. Cancer Res. 56, 5360.
Rajan, J.V., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 13078.
Spillman, M.A. and Bowcock, A.M. 1996. Oncogene 13, 1639.
Stratton, M.R. 1996. Hum. Mol. Genet. 5, 1515.
Tavtigian, S.V., et al. 1996. Nat. Genet. 12, 333.
Vaughn, J.P., et al. 1996. Cancer Res. 56, 4590.
Wooster, R., et al. 1995. Nature 378, 789.产品信息 形式 Liquid 配方 In PBS. 正控制 MCF-7 and HeLa cells and breast carcinoma 防腐剂 ≤ 0.1% sodium azide 应用 主要应用 Immunoblotting (Western Blotting)
Immunoprecipitation
Paraffin Sections
生物信息 免疫原 a recombinant protein consisting of amino acids 3245-3418 of human BRCA2 免疫原品种 Human 宿主 Rabbit 同种型 IgG 品种 human 浓缩标签 Please refer to vial label for lot-specific concentration
储存和货运信息 货运代码 Shipped with Blue Ice or with Dry Ice
毒性 Standard Handling
储存 -20°C
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
特殊指南 Following initial thaw aliquot and freeze (-20°C).
上一件merck millipore产品：merck millipore,默克密理博,MABN730,Anti-Rab10 Antibody, clone 4E2
下一件merck millipore产品：merck millipore,默克密理博,645900,Triptolide, Tripterygium wilfordii - CAS 38748-32-2 - Calbiochem
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