merck millipore,默克密理博,ACS10000,Amnis NFkB Translocation Kit

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Merck Millipore 默克密理愽

描述
产品目录编号	ACS10000
商名	Amnis
描述	Amnis® NFkB Translocation Kit
概述	The Amnis NFkB translocation kit includes the conjugated antibody Anti-Hu NFkB (p50) Alexa Fluor 488, the nuclear stain 7-AAD along with the fixation solution, assay buffer and permeabilization buffer to rapidly detect and quantitate NFkB's translocation from the cytoplasm to the nucleus.
Alternate Names	Amnis Cell Signaling
背景信息	Nuclear Factor kappa B (NFκB) transcription factor plays a central role in regulating many key processes in mammalian cells, including differentiation, cell growth, inflammation, immunity, and stress responses. The p50/p65 heterodimer is considered to be the most abundant form of NFκB. NFκB is held in an inactive state in the cytoplasm by its inhibitor IkB. Phosphorylation of serine residues on the IkB proteins marks them for degradation via ubiquitination, therefore allowing activation of the NFκB complex. Activated NFκB complex translocates into the nucleus. The Anti-Hu NFκB Alexa Fluor® 488 antibody recognizes NFκB p50 which is a component of the NFκB p50/p65 heterodimer.The Amnis NFkB translocation kit includes the conjugated antibody Anti-Hu NFkB (p50) Alexa Fluor® 488, the nuclear stain 7-AAD alonge with the fixation solution, assay buffer and permeabilization buffer. This 2 color imaging flow cytometry kit along with the Amnis imaging cytometry platforms are designed to detect and quantitate NFkB translocation from the cytoplasm to the nucleus.

产品信息
部件	Permeabilization Buffer (Part Number CS202125, 10mL/vial) Anti-Hu NFκB(p50) Alexa Fluor® 488 (Part Number 4700-1674, 50 tests/vial) 7-AAD Reagent (Part Number 4000-0290, 50 tests/vial) 5X Fixation Solution (Part Number 4300-0340, 2-3mL vials) 5X Assay Buffer (Part Number CS202124, 50mL/vial)

应用
应用	The Amnis NFkB translocation kit includes the conjugated antibody Anti-Hu NFkB (p50) Alexa Fluor 488, the nuclear stain 7-AAD along with the fixation solution, assay buffer and permeabilization buffer to rapidly detect and quantitate NFkB's translocation from the cytoplasm to the nucleus.
主要应用	Flow Cytometry

生物信息
品种反应性	Human
抗体类型	Monoclonal Antibody

产品使用声明
质量保证	Lot to lot in-process testing on all kit components
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

产品使用声明

质量保证

Lot to lot in-process testing on all kit components

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

储存和货运信息
存储条件	Anti-Hu NFκB Alexa Fluor® 488, 7-AAD Solution, 5X Assay Buffer, and Permeabilization Buffer at 2-8 °C. Store the 5X Fixation Solution at room temperature.