- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB, ELISA Rb Purified Polyclonal Antibody 描述 产品目录编号 AB3784P Replaces MABS176 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Adiponectin Antibody Alternate Names - GBP28
- Acrp30
- AdipoQ
- apM1
背景信息 Adipose tissue is highly specialized to play important roles in energy storage, fatty acid metabolism, and glucose homeostasis. Excess of adipose tissue in obesity and lack of adipose tissue in lipodystrophic states are associated with insulin resistance and type-2-diabetes in human and animal studies. Several groups have reported the identification of mouse and human transcript encoding an adipocyte-specific secretory protein originally termed Acrp30 (other names include AdipoQ, apM1, GBP28 and adiponectin). Adiponectin is a protein of 247 amino acids consisting of an N-terminal collagen-like domain and a C-terminal globular domain. The globular domain shares significant homology to the globular domains of type VIII and X collagens, subunits of the complement factor C1q as well as hib27, the latter a protein found in the serum of hibernating mice during non-hibernation. The crystal structures for the globular domain of adiponectin at 2.1 Å resolution reveals significant homology to TNF (tumor necrosis factor) family. Adiponectin circulates as a homotimer or larger complexes of 12-18 subunits. The concentration in serum is approximately 5-10 μg/mL, which corresponds to a maximum concentration of 10-30 nM for the higher order complex.
Serum levels of adiponectin are significantly decreased in both obesity and type-2-diabetes, indicating a possible metabolic role or relationship to insulin resistance. In consistence with this, a recent report emphasized a proteolytic fragment (globular domain) of adiponectin to stimulate fatty acid oxidation by muscle.产品信息 格式 Purified 演示 Liquid in PBS, pH 7.4. 应用 应用 This Anti-Adiponectin Antibody is validated for use in ELISA, IP, WB for the detection of Adiponectin. 主要应用 - Western Blotting
- ELISA
应用说明 Western blot: 1:5,000-1:10,000 using ECL. Reacts with the 30 kDa adiponectin protein. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 with 5% skim milk. Suggested incubation time is 1 hour at room temperature.
Typical preparation of blood sample, 1μL of human plasma or serum is used per lane. 1μL of plasma or serum is mixed with SDS-sample buffer, boiled and prepared for protein gel as normal.
Immunoprecipitation: 10 μg in a final reaction volume of 700 μL. Final total protein concentration in the reaction mix: 1.6 mg/mL. Suggested capture agent is Protein A.
ELISA, direct and indirect: 1:5,000-1:10,000.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Recombinant full length human adiponectin from E. coli. 宿主 Rabbit 特异性 Recognizes human adiponectin. Recognizes both natural and recombinant protein. 品种反应性 Human 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_004797.2
Entrez基因汇总 This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. 基因符号 - APM-1
- ADIPOQ
- AdipoQ
- apM-1
- APM1
- GBP28
- ACRP30
- apM1
- adiponectin
- ACDC
UniProt编号 - Q15848
UniProt汇总 FUNCTION: SwissProt: Q15848 # Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
SIZE: 244 amino acids; 26414 Da
SUBUNIT: Homomultimer. Forms trimers, hexamers and 12- to 18-mers. The trimers (low molecular weight complexes / LMW) are assembled via non-covalent interactions of the collagen-like domains in a triple helix and hydrophobic interactions within the globular C1q domain. Several trimers can associate to form disulfide-linked hexamers (middle molecular weight complexes / MMW) and larger complexes (higher molecular weight / HMW). The HMW-complex assembly may rely aditionnally on lysine hydroxylation and glycosylation. LMW, MMW and HMW complexes bind to HBEGF, MMW and HMW complexes bind to PDGFB, and HMW complex binds to FGF2.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Synthesized exclusively by adipocytes and secreted into plasma.DOMAIN:SwissProt: Q15848 The C1q domain is commonly called the globular domain.
PTM: Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting. & HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin- sensitizing activity of adiponectin in hepatocytes (By similarity). & O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. & Not N-glycosylated.
DISEASE: SwissProt: Q15848 # Defects in ADIPOQ are the cause of adiponectin deficiency [MIM:605441]. The result is a very low concentration of plasma adiponectin. Decreased adiponectin plasma levels are associated with obesity insulin resistance, and diabetes type 2.
SIMILARITY: Contains 1 C1q domain. & Contains 1 collagen-like domain.
MISCELLANEOUS: Variants Arg-84 and Ser-90 show impaired formation of HMW complexes whereas variants Cys-112 and Thr-164 show impaired secretion of adiponectin in any form. & HMW-complex blood contents are higher in females than in males, are increased in males by castration and decreased again upon subsequent testosterone treatment, which blocks HMW- complex secretion (By similarity). In type 2 diabetic patients, both the ratios of HMW to total adiponectin and the degree of adiponectin glycosylation are significantly decreased as compared with healthy controls.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 50 µg
上一件merck millipore产品：merck millipore,默克密理博,239785,Cyclophosphamide Monohydrate - CAS 6055-19-2 - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,407951,Ionomycin, Free Acid, Streptomyces conglobatus in Solution - CAS 56092-81-0 - Calbiochem
相关产品
merck millipore,默克密理博,MABE951,Anti-Histone H3.3 Antibody, clone 6C4A3
merck millipore,默克密理博,01-309,PDGF-AA Protein, human
merck millipore,默克密理博,05-23-0850,Somatostatin - CAS 51110-01-1 - Calbiochem
merck millipore,默克密理博,FCIM100158,FlowCellect™ Human CD4/CD8 T Cell Kit
merck millipore,默克密理博,239785,Cyclophosphamide Monohydrate - CAS 6055-19-2 - Calbiochem
merck millipore,默克密理博,ABC276,Anti-CTNS Antibody
merck millipore,默克密理博,ACS10000,Amnis NFkB Translocation Kit
merck millipore,默克密理博,MAB8767-K,Anti-Venezuelan Eq. Encephalitis, Clone 1A3A-9
merck millipore,默克密理博,268320,Daptomycin, Streptomyces sp. - CAS 103060-53-3 - Calbiochem