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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M ICC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABT65 描述 Anti-HAX-1 Antibody Alternate Names - HSP1BP-1
- HCLS1-associated protein X-1
- HS1-associating protein X-1
- HAX-1
- HS1-binding protein 1
背景信息 HAX-1 (HS1-associated protein X-1) is a mitochondrial anti-apoptotic protein and is thought to be regulated by Omi/HtrA2. HAX-1 has been found to associate with BSEP, MDR1 and MDR2 ABC-transporters and interacts with cortactin to play a role in BSEP internalization from the apical membrane. HAX-1 and alphavbeta6 are found to be highly expressed in certain oral cancers and work together to enhance tumor invasion and progression. High expression of HAX-1 in many other cancer tissues shows that HAX-1 is important in neoplastic transformation. 产品信息 格式 Affinity Purified 控制 - Jurkat cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-HAX-1 Antibody is an antibody against HAX-1 for use in WB & IC. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Western Blot Analysis: 0.1 µg/mL from a representative lot detected HAX-1 in 10 µg of HeLa cell lysate and rat liver tissue lysate.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected HAX-1 in NIH/3T3 and HeLa cells.生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to the domain of human HAX-1 that is involved in HCLS1-, CASP9-, and GNA13-bindings. 表位 HCLS1-, CASP9-, and GNA13-binding domains 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes HAX-1 at the domains involved in HCLS1-, CASP9-, and GNA13-binding. 品种反应性 RatHumanMouse Species Reactivity Note Demonstrated to react with Human, Mouse, and Rat. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_006109
Entrez基因汇总 The protein encoded by this gene is known to associate with hematopoietic cell-specific Lyn substrate 1, a substrate of Src family tyrosine kinases. It also interacts with the product of the polycystic kidney disease 2 gene, mutations in which are associated with autosomal-dominant polycystic kidney disease, and with the F-actin-binding protein, cortactin. It was earlier thought that this gene product is mainly localized in the mitochondria, however, recent studies indicate it to be localized in the cell body. Mutations in this gene result in autosomal recessive severe congenital neutropenia, also known as Kostmann disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. 基因符号 - HS1BP1
- HAX1
纯化方法 Affinity Purified UniProt编号 - O00165
UniProt汇总 FUNCTION: Promotes cell survival. Potentiates GNA13-mediated cell migration. Involved in the clathrin-mediated endocytosis pathway. May be involved in internalization of ABC transporters such as ABCB11. May inhibit CASP9 and CASP3. May regulate intracellular calcium pools.
SUBUNIT STRUCTURE: Interacts with ABCB1, ABCB4 and ABCB11 By similarity. Directly associates with HCLS1/HS1, through binding to its N-terminal region. Interacts with CTTN. Interacts with PKD2. Interacts with GNA13. Interacts with CASP9. Interacts with ITGB6. Interacts with PLN and ATP2A2; these interactions are inhibited by calcium.
SUBCELLULAR LOCATION: Mitochondrion. Endoplasmic reticulum. Nucleus membrane. Cytoplasmic vesicle (By similarity). Sarcoplasmic reticulum (By similarity).
TISSUE SPECIFICITY: Ubiquitous. Up-regulated in oral cancers.
PTM: Proteolytically cleaved by caspase-3 during apoptosis.
INVOLVEMENT IN DISEASE: Defects in HAX1 are the cause of neutropenia severe congenital autosomal recessive type 3 (SCN3) [MIM:610738]; also known as Kostmann disease. A disorder of hematopoiesis characterized by maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 109/l and early onset of severe bacterial infections. Some patients affected by severe congenital neutropenia type 3 have neurological manifestations such as psychomotor retardation and seizures. Note=The clinical phenotype due to HAX1 deficiency appears to depend on the localization of the mutations and their influence on the transcript variants. Mutations affecting exclusively isoform 1 are associated with isolated congenital neutropenia, whereas mutations affecting both isoform 1 and isoform 5 are associated with additional neurologic symptoms. Ref.14 Ref.15 Ref.19 Ref.20
SEQUENCE SIMILARITIES: Belongs to the HAX1 family.产品使用声明 质量保证 Evaluated by Western Blot in Jurkat cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected HAX-1 in 10 µg of Jurkat cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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下一件merck millipore产品：merck millipore,默克密理博,ABN88,Anti-phospho-NR1 (Ser896) Antibody
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