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品种反应性 主要应用 宿主 格式 抗体类型 M, R, H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABN88 Replaces 06-640 描述 Anti-phospho-NR1 (Ser896) Antibody Alternate Names - glutamate [NMDA] receptor subunit zeta-1
- glutamate receptor, ionotropic, N-methyl D-aspartate 1
- glutamate [NMDA] receptor subunit zeta 1
- N-methyl-D-aspartate receptor channel, subunit zeta-1
- N-methyl-D-aspartate receptor subunit NR1
背景信息 NR1 (NMDA1or NMDAR1, also known as GRIN1) along with the NR2 subunit forms a heterodimer that make up the NMDA receptor. The NMDA receptor (NMDAR) is characterized as a ionotropic receptor for glutamate. As the name suggests, (NMDA (N-methyl D-aspartate) is an agonist for the receptor. A distinct characteristic of the NMDA receptor is that it is both ligand-gated and voltage-dependent based on its sensitivity to magnesium. Activated NMDA receptors result in the opening of an ion channel that is nonselective to cations. The result is the flow of Na+ and small amounts of Ca2+ ions into the cell and K+ out of the cell. Calcium flux by the NMDARs has been implicated in synaptic plasticity, a mechanism in the cell for learning and memory. 产品信息 格式 Affinity Purified 控制 - Lambda phosphatase untreated and treated rat microsomal brain tissue lysates
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Detect phospho-NR1 (Ser896) using this Anti-phospho-NR1 (Ser896) Antibody validated for use in WB, IH(P). 主要应用 - Western Blotting
应用说明 Immunohistochemistry Analysis: 1:25 dilution from a previous lot detected NR1 in normal rat hippocampus tissue. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to rat NR1 phosphorylated at Ser896. 表位 Phosphorylated Ser896 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes NR1 when phosphorylated at Ser896. 品种反应性 MouseRatHuman Species Reactivity Note Demonstrated to react with rat. Predicted to react with human and mouse based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_058706
Entrez基因汇总 Subunit of NMDA-preferring ionotropic glutamate receptors; may play a role in long term potentiation [RGD]. 基因符号 - NMDA1
- GRIN1
- Glutamate
- NMDAR1
- NR1
修改 - Phosphorylation
纯化方法 Affinity purified UniProt编号 - P35439
UniProt汇总 FUNCTION: NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors.
SUBUNIT STRUCTURE: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B); disulfide-linked (By similarity). Found in a complex with GRIN2A or GRIN2B, GRIN3A and PPP2CB. Found in a complex with GRIN2A or GRIN2B and GRIN3B (By similarity). Interacts with DLG4 and MPDZ. Interacts with LRFN1 and LRFN2.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (By similarity). Cell junction › synapse › postsynaptic cell membrane (By similarity). Cell junction › synapse › postsynaptic cell membrane › postsynaptic density (By similarity). Note: Enriched in post-synaptic plasma membrane and post-synaptic densities (By similarity).
PTM: NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity.
SEQUENCE SIMILARITIES: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily. [View classification]产品使用声明 质量保证 Evaluated by Western Blot in lambda phosphatase untreated and treated rat microsomal brain tissue lysates.
Western Blot Analysis: 1 µg/mL of this antibody detected NR1 on 10 µg of lambda phosphatase untreated and treated rat microsomal brain tissue lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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