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品种反应性 主要应用 宿主 格式 抗体类型 M, R, H, Xn, Ch WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABT135 Replaces 04-974 描述 Anti-phospho-FAK (Tyr397) Antibody Alternate Names - pp125FAK
- Focal adhesion kinase 1
- FADK 1
- Protein-tyrosine kinase 2
背景信息 Focal adhesion kinase (FAK) is a non receptor protein tyrosine kinase discovered as a substrate for Src and a key element in integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK regulation includes phosphorylation at multiple tyrosine and serine residues. Phosphorylation of tyrosine is generally associated with positive regulation and growth promotion; however, dephosphorylation at these sites occurs as cells enter mitosis (M-Phase). In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis and may play a role in focal adhesion disassembly. FAK and its phosphorylation states have been implicated in cancer metastasis, tumor cell survival, and adhesion-independent growth. Recent evidence indicates that elevation of FAK activity in human carcinoma cells is associated with increased invasive potential. A central role in tumor formation and progression suggests that FAK is an attractive target for therapeutic intervention. Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397. 产品信息 格式 Affinity Purified 控制 - untransfected and Src-transfected RAT2 cell lysates
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Detect phospho-FAK (Tyr397) using this Anti-phospho-FAK (Tyr397) Antibody validated for use in WB, Cell Function Assay. 主要应用 - Western Blotting
应用说明 Peptide Inhibition Assay Analysis: A 1:1,000 dilution from a representative lot peptide blocked on RAT2 cells transfected with Src. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human FAK phosphorylated at Tyr397. 表位 Phosphorylated Tyr397 宿主 Rabbit 特异性 This antibody recognizes FAK phosphorylated at Tyr397. 品种反应性 MouseRatHumanXenopusChicken Species Reactivity Note Demonstrated to react with Rat.
Predicted to react with Human, Xenopus, Chicken, and Mouse based on 100% sequence homology.抗体类型 Polyclonal Antibody Entrez基因编号 - NP_722560
Entrez基因汇总 This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only three of them have been determined. [provided by RefSeq]. 基因符号 - FAK1
- PTK2
- FAK
修改 - Phosphorylation
纯化方法 Affinity Purified UniProt编号 - Q05397
UniProt汇总 FUNCTION: Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
SUBUNIT STRUCTURE: Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with RGNEF and SHB By similarity. Interacts with TGFB1I1.
SUBCELLULAR LOCATION: Cell junction › focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note: Constituent of focal adhesions.
TISSUE SPECIFICITY: Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
DOMAIN: The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL By similarity.
The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
PTM: Phosphorylated on 6 tyrosine residues upon activation.
SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain.产品使用声明 质量保证 Evaluated by Western Blot in untransfected and Src-transfected RAT2 cell lysates.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected phospho-FAK (Tyr397) in 10 µg of untransfected and Src-transfected RAT2 cell lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µL
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