merck millipore,默克密理博,71464,pET-50b(+) DNA

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merck millipore,默克密理博,71464,pET-50b(+) DNA - Novagen
产品名称：pET-50b(+) DNA - Novagen
产品型号：71464
Novagen's pET-50b(+) vector is designed for cloning and high-level expression of target proteins fused with the 495 aa NusA (Nus-Tag) protein that is cleavable with the human rhinovirus 3C protease.

merck millipore,默克密理博,71464,pET-50b(+) DNA - Novagen

产品介绍

merck millipore,默克密理博,71464,pET-50b(+) DNA - Novagen

描述
概述	The pET-50b(+) vector is designed for cloning and high-level expression of target proteins fused with the 495 aa NusA (Nus•Tag™) protein that is cleavable with the human rhinovirus (HRV) 3C protease (1). The NusA protein has been identified as having the highest potential for solubility when a data base of more than 4000 E. coli proteins was subjected to solubility modeling (2,3). Greater than 85% of the expressed protein was soluble in tests with each of four different NusA fusion proteins (2). Cloning sites are also available for producing fusion proteins containing two cleavable N-terminal His•Tag^® sequences for detection and purification. The additional N-terminal His•Tag sequence significantly enhances purification yields when utilizing His•Mag™ Agarose Beads for high throughput purification (4). The plasmid contains a strong T7lac promoter, optimized RBS, the coding sequence for the HRV 3C protease cleavage site (LeuGluValLeuPheGlnGlyPro) for N-terminal fusion tag removal and a multiple cloning site that encodes restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional C-terminal S•Tag™ coding sequence is compatible with purification, detection, and quantification (5). The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information. Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
产品目录编号	71464
品牌系列	Novagen®

参考
参考	1. Walker, P.A., Leong L. E.-C., Ng, P.W.P., Tan, S.H.,Waller, S., Murphy, D. and Porter, A.G. (1994) Bio/Technology 12, 601-605. 2. Harrison, R.G. (2000) inNovations 11, 4-7. 3. Davis, G.D., Elisee, C., Newham, D.M. and Harrison, R.G. (1998) Biotechnol. Bioeng. 65, 382-388. 4. Novy, R. and Drott, D. (2002) inNovations 14, 12-13. 5. Kim, J.S. and Raines, R.T. (1993) Protein Science 2, 348-356.

参考

1. Walker, P.A., Leong L. E.-C., Ng, P.W.P., Tan, S.H.,Waller, S., Murphy, D. and Porter, A.G. (1994) Bio/Technology 12, 601-605.

2. Harrison, R.G. (2000) inNovations 11, 4-7.

3. Davis, G.D., Elisee, C., Newham, D.M. and Harrison, R.G. (1998) Biotechnol. Bioeng. 65, 382-388.

4. Novy, R. and Drott, D. (2002) inNovations 14, 12-13.

5. Kim, J.S. and Raines, R.T. (1993) Protein Science 2, 348-356.