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merck millipore,默克密理博,ABS223,Anti-RAS-Related protein Ral-A Antibody
产品名称：Anti-RAS-Related protein Ral-A Antibody
产品型号：ABS223
This Anti-RAS-Related protein Ral-A Antibody is validated for use in Western Blotting for the detection of RAS-Related protein Ral-A.
merck millipore,默克密理博,ABS223,Anti-RAS-Related protein Ral-A Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABS223 Replaces 07-2123 描述 Anti-RAS-Related protein Ral-A Antibody Alternate Names - Ras-related protein Ral-A
背景信息 RalA is a Ras-family GTPase which has a well-documented role in tumorigenesis. RalA is activated by Ras GTPases and in turn interacts with a number of effector molecules, most notably the exocyst or Sec6/8 complex . The Sec6/8 complex is an octameric structure which plays a key role in exocytosis of secretory vesicles by forming a bridge between vesicular and plasma membranes. RalA indirectly mediates this process as it is involved in the assembly of Sec6/8; RalA interacts with components of the Sec6/8 complex including Exo84 and Sec5. RalA may promote tumorigenesis through a Ras-induced pathway involved in cell transformation, actin dynamics or exocytosis. 产品信息 格式 Affinity Purified 控制 - HeLa cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 This Anti-RAS-Related protein Ral-A Antibody is validated for use in Western Blotting for the detection of RAS-Related protein Ral-A. 主要应用 - Western Blotting
生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to the C-terminus of human RAS-Related protein Ral-A. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 品种反应性 Human Species Reactivity Note Demonstrated to react in human. Predicted to react with Rat, Mouse, Equine, Porcine, and Bovine based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_005393
Entrez基因汇总 The product of this gene belongs to the small GTPase superfamily, Ras family of proteins. GTP-binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors. This gene encodes a low molecular mass ras-like GTP-binding protein that shares about 50% similarity with other ras proteins. [provided by RefSeq, Jul 2008]. 基因符号 - RAL
- RALA
纯化方法 Affinity purified UniProt编号 - P11233
UniProt汇总 FUNCTION: Multifuntional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Plays a role in the early stages of cytokinesis and is required to tether the exocyst to the cytokinetic furrow. The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling. Key regulator of LPAR1 signaling and competes with ADRBK1 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells.
ENZYME REGULATION: Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).
SUBUNIT STRUCTURE: Interacts with RALBP1 via its effector domain. Interacts with EXOC8 and EXOC2. EXOC2 and EXOC8 have overlapping binding sites and compete for RALA binding. Interacts with Clostridium exoenzyme C3. Interacts with RALGPS1. Interacts with LPAR1 and LPAR2. Interacts with ADRBK1 in response to LPAR1 activation. RALA and ADRBK1 mutually inhibit each other's binding to LPAR1.
SUBCELLULAR LOCATION: Cell surface. Cell membrane; Lipid-anchor; Cytoplasmic side. Cleavage furrow. Midbody. Note: Prior to LPA treatment found predominantly at the cell surface and in the presence of LPA co-localizes with LPAR1 and LPAR2 in the endocytic vesicles. During early cytokinesis localizes at the cleavage furrow membrane. Colocalizes with EXOC2 at the early midbody ring and persists there till maturation of the midbody.
INDUCTION: Activated in an LPA-dependent manner by LPAR1 and in an LPA-independent manner by LPAR2.
PTM: Prenylation is essential for membrane localization. The geranylgeranylated form and the farnesylated mutant does not undergo alternative prenylation in response to geranylgeranyltransferase I inhibitors (GGTIs) and farnesyltransferase I inhibitors (FTIs).
SEQUENCE SIMILARITIES: Belongs to the small GTPase superfamily. Ras family.产品使用声明 质量保证 Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected RAS-Related protein Ral-A on 10 µg of HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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